FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n01_Fluc_2554.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n01_Fluc_2554.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5093734
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAATAC51650.020.9170553
TAGGTAG25250.020.6754865
GTATTAG47200.020.1457371
TATACAG67300.019.7160845
TACTATG52500.016.709027
TATACTG62750.016.2925855
CTATGCA43800.016.0554529
GTATATA125750.015.9314371
GTATAGA49950.015.6943061
TATATAC141550.015.4184683
ACTATGC52750.015.3929018
TATTAGG55400.015.3295692
TAATACT96900.015.1899684
CCTAATA54600.014.889472
TCTATAC46850.014.8575173
TAGTCAG27250.014.6325879
ACCTAAT56400.014.2855761
ATACAGG46050.014.0115216
GTATAAT65900.013.7682411
GTATTAT92900.013.5952561
Produced by FastQC (version 0.11.5)