FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n01_Fluc_2553.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n01_Fluc_2553.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3194053
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG13900.022.949885
TATACAG47700.021.735035
TATACCG20100.019.8385165
GTATATA82950.019.0747491
GTATTAG26000.018.9825381
TACAGGC20800.018.1246227
ATACAGG34600.017.8103226
TATATAC96500.016.8291913
TATACTG42350.016.2634535
TATACGG17050.016.1585275
ACTGCGT10150.015.7139588
CTAATAC31450.015.2147853
GTATAAG24850.014.3116061
TCTATAC31150.014.197583
GTATAGA33900.014.1306771
GGACCGT5658.367424E-714.114756
ACAGGCA26800.014.0668718
TCTACAC21350.013.9228623
TATAAGG28250.013.8737552
TGGACCG7851.0095391E-913.8536475
Produced by FastQC (version 0.11.5)