FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n01_Fluc_2552.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n01_Fluc_2552.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2487286
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATATATACAGGCAACACGCAGATATAGGTGCGACGTGAACAGTGAGCTG27250.10955716391279492No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG10450.027.7536685
GTATTAG20450.025.2007481
TACAGGC18750.025.1325387
ATACAGG30300.024.1659036
TATACAG40800.023.6356835
ACAGGCA22150.020.9474188
CTAATAC24950.019.7612783
GTATATA71650.018.5388721
CTTACAC10700.018.2960223
CAGCGTG8100.017.900679
TTAGGTA19450.017.520844
TACTATG31100.017.250267
TAGTCAG17100.016.9585299
CAGGCAA27000.016.9161329
TCTATAC26250.016.849123
GTATAAT30400.016.7137091
TATATAC80150.016.5548113
ATATACA72950.016.3002854
TACAGGT18550.016.0237527
ATACTAT47150.015.9910116
Produced by FastQC (version 0.11.5)