FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n01_Fluc_2551.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n01_Fluc_2551.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10402538
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATATAGGTGCGACGTGAACAGTGAGCTGTATGTGCGCAGCTCGCGTTGC248350.2387398152258612No Hit
TATATATACAGGCAACACGCAGATATAGGTGCGACGTGAACAGTGAGCTG237150.22797321192193676No Hit
GTATGTGCGCAGCTCGCGTTGCATTTTCGGAAGCGCTCGTTTTCGGAAAC213870.20559405791163657No Hit
GTATAGGAACTTCAGAGCGCTTTTGAAAACCAAAAGCGCTCTGAAGACGC146920.14123476405469512No Hit
TTACAGTCCGGTGCGTTTTTGGTTTTTTGAAAGTGCGTCTTCAGAGCGCT146350.14068682085083467No Hit
CTATATAACCTACCCATCCACCTTTCGCTCCTTGAACTTGCATCTAAACT143860.1382931742234443No Hit
ATCCCATTCCATGCGGGGTATCGTATGCTTCCTTCAGCACTACCCTTTAG116950.11242448717803291No Hit
GTACAAATAATGCGCGGCCGGTGCATTTTTCGAAAGAACGCGAGACAAAC109480.10524354729586184No Hit
CTTCAAAGCGTTTCCGAAAACGAGCGCTTCCGAAAATGCAACGCGAGCTG107690.10352281337496676No Hit
TTCCTATACTTTCTAGAGAATAGGAACTTCGGAATAGGAACTTCAAAGCG104450.10040818884776002No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACAGGC102700.035.507257
ACAGGCA111950.032.443918
ATACAGG131700.032.037366
TATACAG139950.031.8097955
GGTGCGA138750.025.602517
GTGCGAC138800.025.4888258
AGGTGCG145350.024.7891046
TATAGGT155650.023.8498843
CAGGCAA189800.020.0531489
ATACTAT110900.019.67686
ATAGGTG188250.019.6041584
TATATAC228050.019.3621083
CCAGGAT78400.019.0509473
CCGCGCG11800.019.0457979
CTAACAG57700.018.9743353
TAATGCG80600.018.6189338
TGCGACG191450.018.4792339
ATATACA258500.018.4276624
GTGCGCA184700.018.4107465
GCGCAGC186200.018.338377
Produced by FastQC (version 0.11.5)