FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n01_Fluc_2550.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n01_Fluc_2550.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27522404
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG130900.029.6337875
GTATTAG252550.024.9486331
TATACAG356300.021.3467965
TAGTCAG168150.020.9105689
CTAATAC287450.020.2294963
ATACAGG256950.018.5369246
TTAGGTA222600.018.4033264
GTAGTCA196750.018.0920478
TACAGGC156250.017.6313087
GTATAGA268450.017.4680121
GTATATA652100.017.4544531
CCTAATA300300.017.4282722
CTATGCA271000.016.9338369
TATTAGG303050.016.7192022
GGTAGTC223600.016.6652667
TACTATG322250.016.5129287
ATACTAT450650.016.3928996
ACTATGC347850.016.2980528
ACCTAAT292050.016.081311
ATTAGGT275800.015.6947013
Produced by FastQC (version 0.11.5)