FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n01_Fluc_2549.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n01_Fluc_2549.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11439112
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACAG156100.019.135715
CTAATAC117050.018.4584473
TATACTG136200.016.5551665
GTATTAG100000.015.8157461
TATATAC287100.015.7075133
TAATACT204800.015.1871974
TAGGTAG56550.015.0004235
GTATATA268700.014.9310621
CTTACAC60450.014.8722143
GTATAGA115550.014.2524361
TATACGG78450.014.2323955
TCTATAC109100.014.0219363
TATACCG70650.013.7512725
CATATAC158150.013.7073113
ACTATGC115900.013.6365448
CCTAATA133000.013.3643442
TACAGCG57100.013.3316677
ATACAGG109500.013.3080136
CTATACA112900.013.2931124
TACACTG76250.013.1216815
Produced by FastQC (version 0.11.5)