FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n01_Fluc_2548.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n01_Fluc_2548.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15294773
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG72400.022.4321885
GTATTAG134800.020.514341
TATACAG197450.018.8374635
CTAATAC150600.017.8130763
GTATAGA149450.016.4636551
GTATATA367050.016.1356831
TAGTCAG85150.016.0064729
ACTATGC150250.015.9228898
TATACTG189300.015.8949685
GGTAGTC98100.015.7410267
TATATAC389650.015.5744583
TCTATAC135400.015.3682863
TATTAGG165350.014.7049342
GACTAGG47700.014.5906728
TTAGGTA141900.014.2044564
GTGTAAG82050.014.1535011
CTATGCA130650.013.9834259
TACTATG166550.013.972787
TATACCG86550.013.9060325
CCTAATA163650.013.881962
Produced by FastQC (version 0.11.5)