FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n01_Fluc_2547.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n01_Fluc_2547.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1224060
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTCGC1409.998374E-425.8917488
GTATTAG9150.023.7908541
TAGGTAG4307.094059E-1121.9194565
TATACCG7400.021.5548925
GTATAGA12100.020.389351
GTGTAGG4001.6165359E-819.9545781
GTATATA29150.018.9184231
TACACAG6900.018.9137785
TAGGACG2701.0790987E-418.7970284
GTCTATA8650.018.45511
CCTAATA11600.018.1405262
CTAATAC11850.017.7433053
GTATATG16450.017.6442811
TATACGG7500.017.4006775
TACTCGG3352.858115E-517.3141065
ACCGTGC3352.8601207E-517.312698
CGGTCAG2952.1269164E-417.2026549
TCTATAC11500.017.02243
TATACAG16000.016.7662775
ATAGGAC6104.2564352E-1016.6399923
Produced by FastQC (version 0.11.5)