FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n01_Fluc_2546.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n01_Fluc_2546.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18680259
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAATACTATGCAAAAATGTAAAATCATCACACAAAACATAAACAATC198670.10635291512821102No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG84500.027.6285115
GTATTAG169550.022.306241
TATACAG261800.022.1830465
ATACAGG194500.020.6122536
CTAATAC213700.020.3905183
TACAGGC123900.019.8357357
TTAGGTA137900.018.980224
ATACTAT312700.018.8487536
TAGTCAG124250.018.84639
TACTATG239600.018.7596537
CCTAATA209650.018.7322032
CTATGCA203900.017.955369
GTATATA420250.017.8261851
GTATAGA190650.017.4768141
ACCTAAT201100.017.3988841
TATATAC475950.016.954783
ACTATGC278550.016.8391868
GTAGTCA137600.016.6490178
GTATAAT202000.016.3510821
TAATACT387550.016.3322054
Produced by FastQC (version 0.11.5)