FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n01_Fluc_2545.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n01_Fluc_2545.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64656365
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACAG716800.018.2981155
GTATTAG517100.018.263051
TAGGTAG270650.017.2521655
CTAATAC591150.016.2511443
GTATAGA561350.015.9182331
GTATATA1240750.015.6439951
TATACGG360100.015.0002645
TCTATAC531750.014.9577033
TATACCG337200.014.4063185
ACTATGC558100.014.392958
TATACTG734050.014.3616565
TATATAC1356650.014.2588253
ATACAGG518200.014.0042146
CATATAC737300.013.58048253
CTAGGAC175450.013.2239873
GTGTAAG302850.013.0389261
GTATATG742200.013.0370621
CCTAATA644100.012.8588722
TATTAGG629700.012.6918282
TAATACT1039700.012.5315794
Produced by FastQC (version 0.11.5)