FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n01_Fluc_2544.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n01_Fluc_2544.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17111600
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAATAC155150.017.8049583
GTATTAG134950.017.5348741
TAGGTAG73700.017.4135
GTATATA302400.016.3944511
CTATGCA128250.016.28029
ACTATGC151050.016.2706018
GTATAGA143100.015.9782371
TATACAG179250.015.7346925
TACTATG162350.014.6022627
CCTAATA157600.014.6002742
TATATAC325550.014.4987683
ATACTAT232800.014.2940186
TAATACT261000.014.2509774
GTGTAAG78550.014.2309031
TATACTG178300.014.1512785
TCTATAC136000.013.9678693
TATTAGG147700.013.662252
TATACCG89800.013.6451445
TAGACAG98100.013.5993015
TATACGG85200.013.3606585
Produced by FastQC (version 0.11.5)