FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n01_Fluc_2543.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n01_Fluc_2543.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51583117
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGA378900.016.2448351
GTATTAG359650.016.2061371
CTAATAC421550.015.686013
GTATATA739750.015.4932011
TAGGTAG196550.015.4194895
ACTATGC373000.015.1991768
TATACAG435650.014.8454685
TATACCG238950.014.564685
TATACGG249650.014.5503315
CTAGGAC139500.014.2410873
CTATGCA307900.014.1509939
CCTAATA415850.014.0334152
TCTATAC381550.013.7769573
TATATAC794150.013.5304553
CTATACT376650.013.43643954
TAGACAG278600.013.4027175
TATACTG523700.013.4016895
TAATACT695150.013.3400914
GTATAGG293700.013.2218991
TACACCG154100.013.1741365
Produced by FastQC (version 0.11.5)