FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n01_Fluc_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n01_Fluc_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4188222
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG30400.020.7740021
TATACAG39400.018.034165
CTAATAC35500.017.972993
TAGGTAG16200.017.454835
GTATATA68100.016.7350521
ACTATGC39100.016.316638
CTATGCA29350.016.0557219
ATAATAC57600.015.9862763
ATACAGG27300.015.6680156
TATATAC76150.015.4245013
TATAATA82600.015.2912752
GTATAGA34450.015.1711241
TAATACT57950.014.8887954
ATTATAC46550.014.6411173
TACACCG12900.014.6133465
ATATACA67850.014.5329934
TATACGG20500.014.50093655
TATACCG19600.014.4269515
TACAGGC17600.014.4171677
TACTATG37250.014.4022397
Produced by FastQC (version 0.11.5)