FastQCFastQC Report
Thu 17 Dec 2020
HNYY3DRXX_l01_n01_Fluc_1773.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYY3DRXX_l01_n01_Fluc_1773.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5277879
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACAG68250.020.6103345
ATACAGG50850.020.5301276
TACAGGC32250.018.8829427
TAGGTAG20400.017.771575
GTATATA117200.017.0338941
GTATTAG44950.016.7962571
TATACGG30700.016.7689515
ACAGGCA36500.015.8897558
GTATTAA51800.015.2758581
TATACCG28100.014.7080285
ATATACA123950.014.6829434
ATTATAC73250.014.5510943
GTATAAT63900.014.5417941
TATATAC131800.014.4685933
TATTATA126650.014.3872362
TTATACT59550.014.1241454
ATACGGT34450.014.0995656
CAGGCAA45900.013.26742659
TTAGGTA36100.013.25630954
TATAATA121600.013.2534282
Produced by FastQC (version 0.11.5)