FastQCFastQC Report
Mon 29 Jan 2024
HNYVCDMXY_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYVCDMXY_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3640317843
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATT703831401.9334339207588802No Hit
GCCCCAATCGGGCGGTAAATTCCGTCCAAGGCTAAATACGGGCGAGAGAC209342780.5750673128791408No Hit
GTTTGGGAATGCAGCCCCAATCGGGCGGTAAATTCCGTCCAAGGCTAAAT194647430.5346989971611662No Hit
GTTTGGGAATGCAGCCCAAATCGGGCGGTAAACTCCGTCCAAGGCTAAAT190607360.5236008728373007No Hit
GGCTAAATACGGGCGAGAGACCGATAGCGAACAAGTACCGCGAGGTAAAA184808980.5076726482973756No Hit
GCCCAAATCGGGCGGTAAACTCCGTCCAAGGCTAAATACAGGCGAGAGAC144439110.3967760954657937No Hit
GCTAAATACAGGCGAGAGACCGATAGCGAACAAGTACCGCGAGGGAAAGA138122670.3794247534335424No Hit
GGCTAAATACGGGCGAGAGACCGATAGCGAACAAGTACCGCGAGGTAAAG127672060.3507167931654698No Hit
GTCCAAGGCTAAATACGGGCGAGAGACCGATAGCGAACAAGTACCGCGAG126590580.34774595367660593No Hit
ATGCAGCCCCAATCGGGCGGTAAATTCCGTCCAAGGCTAAATACGGGCGA115400840.31700759377894794No Hit
GTCCAAGGCTAAATACAGGCGAGAGACCGATAGCGAACAAGTACCGCGAG113605240.31207505745261377No Hit
AACAAGTACCGCGAGGGAAAGATGAAAAGGACTTTGAAAAAAAAAAAAAA101461860.27871703619260035No Hit
GTAAATTCCGTCCAAGGCTAAATACGGGCGAGAGACCGATAGCGAACAAG100116200.27502049084124436No Hit
CAAGTACCGCGAGGGAAAGATGAAAAGGACTTTGAAAAAAAAAAAAAAAA99094630.2722142248939882No Hit
GGCTAAATACAGGCGAGAGACCGATAGCGAACAAGTACCGCGAGGGAAAG88326890.24263510443145667No Hit
GCTTAAACTCAGCGGGTAATCCCGCTGAGTTTAAGCATATCAAAAAAAAA76242950.20944036561699758No Hit
GCTAAATACGGGCGAGAGACCGATAGCGAACAAGTACCGCGAGGTAAAAA73355770.20150924497171716No Hit
TGCTTAAACTCAGCGGGTAATCCCGCTGAGTTTAAGCATATCAAAAAAAA72044910.19790829566856588No Hit
GACCGATAGCGAACAAGTACCGCGAGGTAAAAAAAAAAAAAAAAAAAAAA71943580.19762994085349156No Hit
ATTCCGTCCAAGGCTAAATACGGGCGAGAGACCGATAGCGAACAAGTACC70548840.1937985721100123No Hit
ATACAGGCGAGAGACCGATAGCGAACAAGTACCGCGAGGGAAAGATGAAA70546590.19379239133103357No Hit
AAATACGGGCGAGAGACCGATAGCGAACAAGTACCGCGAGGTAAAAAAAA69758710.19162807482357522No Hit
AAATACAGGCGAGAGACCGATAGCGAACAAGTACCGCGAGGGAAAGATGA66749760.1833624504199646No Hit
GGTAAATTCCGTCCAAGGCTAAATACGGGCGAGAGACCGATAGCGAACAA63933500.17562614792809453No Hit
GACCGATAGCGAACAAGTACCGCGAGGGAAAGATGAAAAGGACTTTGAAA61030410.1676513223079021No Hit
CTTAAACTCAGCGGGTAATCCCGCTGAGTTTAAGCATATCAAAAAAAAAA57061180.1567477963764166No Hit
GATGAAAAGGACTTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA56968000.15649182971630976No Hit
GCGCTGTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGT55977880.15377195732411217No Hit
CCACGAGGCGCCGTCAACGAGTCGGGTTGTTTGGGAATGCAGCCCAAATC52735360.1448647131222492No Hit
GTAAACTCCGTCCAAGGCTAAATACAGGCGAGAGACCGATAGCGAACAAG52666440.14467538899459775No Hit
GTACTTGTTCGCTATCGGTCTCTCGCCCGTATTTAGCCTTGGACGGAATT52399010.14394075534024736No Hit
CTGTCGCACCACGAGGCGCTGTCTACGAGTCGGGTTGTTTGGGAATGCAG51192690.14062697876351343No Hit
GCTAAATACGGGCGAGAGACCGATAGCGAACAAGTACCGCGAGGTAAAGA51155480.14052476241426923No Hit
CCACGAGGCGCTGTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATC50891740.13980026523744402No Hit
GGTAAACTCCGTCCAAGGCTAAATACAGGCGAGAGACCGATAGCGAACAA48212690.13244088038276278No Hit
CCGTCAACGAGTCGGGTTGTTTGGGAATGCAGCCCAAATCGGGCGGTAAA45271630.12436175068353778No Hit
CTGTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAA44479800.12218658347520563No Hit
GTCGGGGGCATTCGTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATG44255130.12156941209158038No Hit
GGCCTAAGTTCCCTGGAAAGGGGCGCCAGAGAGGGTGAGAGCCCCGTCGT42828240.11764972688402692No Hit
ACTCCGTCCAAGGCTAAATACAGGCGAGAGACCGATAGCGAACAAGTACC42405000.11648708115292998No Hit
CTGTCGCACCACGAGGCGCCGTCAACGAGTCGGGTTGTTTGGGAATGCAG41561210.1141691791553818No Hit
GCCCGGACCCTGTCGCACCACGAGGCGCCGTCAACGAGTCGGGTTGTTTG40520190.11130948380762037No Hit
GTCGGGTTGTTTGGGAATGCAGCCCAAATCGGGCGGTAAACTCCGTCCAA39147490.10753865922800412No Hit
GGCGAGAGACCGATAGCGAACAAGTACCGCGAGGGAAAGATGAAAAGGAC38803510.10659374173773209No Hit
CCGTCGTGCCCGGACCCTGTCGCACCACGAGGCGCTGTCTACGAGTCGGG38433630.10557767661388243No Hit
ATGCAGCCCAAATCGGGCGGTAAACTCCGTCCAAGGCTAAATACAGGCGA38030110.10446920197676816No Hit
GGAAAGATGAAAAGGACTTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA36824600.10115765048046657No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)