FastQCFastQC Report
Mon 29 Jan 2024
HNYVCDMXY_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYVCDMXY_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3640317843
Sequences flagged as poor quality0
Sequence length10
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCGTAGTT757813572.081723637009352No Hit
GGGGGGGGGG673690751.8506371670139903No Hit
CTACTAATAA648059781.7802285623112826No Hit
TAAGTCCTGA646232871.7752100170116931No Hit
CCGTATGATT643467961.7676147736311827No Hit
GTAGCAGCTA598953151.6453320172350676No Hit
ACTATTCATT597438791.6411720508109489No Hit
CCAGATACGG591491861.624835757507782No Hit
TTGATACGAT582491921.600112806413536No Hit
CGTTACCGCT582206791.5993295506312195No Hit
AATATTACTT571616771.5702386292976231No Hit
TCGCCGCCGG568505091.561690804260907No Hit
TTGCGAGCAT564509391.5507145648985026No Hit
CGTCGAGCGC553208181.5196699954751727No Hit
TTCGAATATT550413791.511993770154976No Hit
TGGACGACTA542914731.4913937557512338No Hit
GACTGGACCA515638751.416466287391708No Hit
TCTTAGCCTG477477381.3116365125043836No Hit
AATACGATAA477454941.3115748695353688No Hit
CCTCAGCTCC475417961.3059792592401938No Hit
CGAATGGAGC475017221.3048784212988844No Hit
TCTATCGCTG468268061.286338391853439No Hit
TGCGACCTCT464354441.2755876273081797No Hit
ATTCTTAGCT464262741.2753357262271343No Hit
TATAATAGTT459120041.2612086630920047No Hit
CAGCTAGCGG456651171.2544266454043254No Hit
AGGTCAGCTT455883261.2523171867440697No Hit
TATTAGACTT454914171.2496550840327267No Hit
GCGTTCCGTT425300681.168306445597366No Hit
ACGGCGTTAA421263661.1572166996627826No Hit
GCTAACGGAT413545361.1360144301553505No Hit
GAGCGCGATG412630191.1335004463784675No Hit
CATTATTATT411554241.130544797870827No Hit
ATTATGCAAG409950641.1261396880173464No Hit
CTCGTCGTAG403134461.1074155537687207No Hit
CTATATAGCC402349831.1052601650531206No Hit
TCTTGAGGTT401337601.1024795562061585No Hit
CTAATTGCGA400191261.0993305454619338No Hit
TCAATCTCCT398964701.0959611693445197No Hit
GCGCTATGGT395504981.086457273945241No Hit
CTAACTAGGT382018801.049410563790707No Hit
TAGTAAGCCG373489091.025979340562763No Hit
GGCTTACTCC364044871.0000359465864366No Hit
AAGTAGCTCA363455740.9984175988887684No Hit
AGACCATATC359816830.9884214662516214No Hit
TTGGCAAGCC359103370.9864615824426515No Hit
GCATCGTATG358482530.984756126966576No Hit
AGAGGAGAAT353116150.9700146119905717No Hit
ATACCTCGAC350155070.9618804870935003No Hit
ATAAGGACTC345794610.9499022473131887No Hit
AGTCCGCTGC343624210.943940130559638No Hit
ACCAGGCGCA341319390.9376087603348321No Hit
ATTGGCAGAT340566390.9355402596366089No Hit
CTAAGCCTTG338959230.9311253704172776No Hit
AAGTACGCGA334578300.9190908992833239No Hit
TAGGTAACTT333150050.9151674781382544No Hit
TGGTTGCCGT332397360.9130998290140238No Hit
GATCTTCGCA330810990.9087420501924561No Hit
GTACTCCTAT326414260.8966641762550073No Hit
TATTGCTGGA325951770.8953937102683921No Hit
AACGAATTCG324061360.8902007296509575No Hit
TTATTGAGGC322295620.8853502191292038No Hit
CTCAATTAGT317545970.8723028694063405No Hit
TCCGGCTTAT316601950.8697096343078853No Hit
GATTCTTGAA298410950.8197387230178735No Hit
TTGCTTAGAT276786830.7603369868711763No Hit
TTGATTGGCG273969280.7525971407326918No Hit
AGTTCTCATG264924530.7277510959913178No Hit
GTAGGATAAG261600060.7186187340839841No Hit
TTGGTAAGGA261380850.7180165613906808No Hit
GTAGGCTGCA260687330.716111453018527No Hit
GCATATGAGC260311580.715079262929075No Hit
AACGCGCAGA252102610.6925291166120848No Hit
CTAGCCAGCC243037920.6676282964338947No Hit
TAGAGGCGTT236302090.6491248846701323No Hit
GCTTGAAGAG233892250.642505023152727No Hit
TGATTAGGTA231621230.6362665019632463No Hit
GCGGCTGCGG230288250.6326047887352017No Hit
CCGCTAAGAG228459200.6275803648280499No Hit
TCGGATTCGG224474800.6166351667111832No Hit
AGTTGAATCA222353160.6108069943056343No Hit
AGATTACGTT221342830.6080316047831431No Hit
TGATATTGCG204114900.56070625918694No Hit
ATGGCGCCTG200840330.5517109732222907No Hit
TTGCCGTAGG170503850.46837627194521875No Hit
TCGTAGCATC164232990.45115013876001264No Hit
GCAAGACCGT145790340.4004879416788881No Hit
CGCGGCCATA143256250.39352676381121154No Hit
TTCCGTCGAC130866190.3594911094140963No Hit
CATAGCGACC114698310.31507773482075035No Hit
GCGCGTTCAT90029110.24731112469510813No Hit
CCGCTACGAG81578230.22409644849245106No Hit
GCTTGACGAG79335100.21793454149217814No Hit
TCCTCTGCCG41604170.11428719082868281No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)