FastQCFastQC Report
Wed 2 May 2018
HNYJ5BGX5_n04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYJ5BGX5_n04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences502573517
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG37047780.7371614051840301No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA817950.014.75318194-95
TCGCCGT1170000.012.73161494-95
TGGTCGC2227900.012.46610294-95
GTGGTCG2956850.011.43141394-95
GTCGCCG1669650.011.35259594-95
GGTCGCC2172950.010.8773794-95
TCGGTGG5006200.010.86828994-95
GCCGTAT886050.010.43224794-95
GATCTCG7809850.010.42099994-95
ATCTCGG7157150.010.32838794-95
CTCGGTG5786000.010.31683494-95
TCTCGGT6347550.010.25908794-95
CGGTGGT4752900.09.53816994-95
TAGATCT10845600.08.84383294-95
CCGTATC828050.08.45253294-95
AGATCTC11093150.08.18595794-95
GTGTAGA15777750.08.18091394-95
GTAGATA1850000.07.915804494-95
CGTATCA772000.07.82641894-95
GGTGGTC5582250.07.33442594-95
Produced by FastQC (version 0.11.2)