FastQCFastQC Report
Wed 2 May 2018
HNYJ5BGX5_n03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYJ5BGX5_n03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences502573517
Sequences flagged as poor quality0
Sequence length8
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATGAGT9843488319.586165937987538No Hit
AGGATAGG9416099218.73576478165283No Hit
ACGAATAA9089488318.0858879199558No Hit
TCAGAGCC8545359617.003203135353427No Hit
CATCCGAA8542868116.998245651690397No Hit
AGATCTCG174942793.4809392871371694Illumina Single End PCR Primer 1 (100% over 8bp)
TCAGCGCC30532850.6075300223191028No Hit
GGGGGGGG17872570.35562100658797746No Hit
ACAGAGCC6674950.1328153946480232No Hit
ATATGAGT6190100.12316804985965865No Hit
TAAGAGCC5147260.10241805081026584No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)