FastQCFastQC Report
Wed 2 May 2018
HNYJ5BGX5_n02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYJ5BGX5_n02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences502573517
Sequences flagged as poor quality0
Sequence length8
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATTCGT9888035219.674803517352863No Hit
TCCGGAGA9504706318.91207152485116No Hit
CTGAAGCT8956121317.82051977880084No Hit
AGCGATAG8823873217.557377978593326No Hit
CGTAGCTC8514137416.94107849299986No Hit
AAAAAAAT139293082.7715961006357603No Hit
GGGGGGGG72577261.444112304867031No Hit
TCCGGAGC9445160.1879358875967195No Hit
CGTAGCTA8282920.16481011672566903No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)