FastQCFastQC Report
Wed 2 May 2018
HNYJ5BGX5_n01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYJ5BGX5_n01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences502573517
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13043570.25953556164002967No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG4168600.015.39116774-75
GCTTGAA4611600.014.93111360-61
CCGTCTT4707900.013.91470852-53
CGTCTTC5034850.013.419682552-53
GCCGTCT5031250.013.34867850-51
AAGGGGG5036350.012.54976174-75
CTTGAAA5622600.012.14956162-63
TGCTTGA5556650.012.06419460-61
AAAAGGG5452000.011.75728472-73
AAAGGGG5561150.011.71355572-73
ATGCCGT5892550.011.63740448-49
TGCCGTC6160100.010.90983450-51
CTGCTTG6459800.010.74275758-59
TATGCCG6274850.010.67327148-49
TCGTATG8622800.09.89117794-95
CGTATGC7898550.09.8815694-95
TTGAAAA7680000.09.58216462-63
AAAAAGG7376150.09.236649570-71
CTCGTAT8812750.09.1668794-95
GTATGCC7848050.09.00542294-95
Produced by FastQC (version 0.11.2)