FastQCFastQC Report
Thu 18 Mar 2021
HNYGNAFX2_n01_AH8967-3hr-spikein.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNYGNAFX2_n01_AH8967-3hr-spikein.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22801841
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTAT4753352.0846343065018305TruSeq Adapter, Index 7 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCGCGTAT293000.12849839624791698TruSeq Adapter, Index 7 (97% over 36bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG239900.10521080293472795No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)