FastQCFastQC Report
Wed 12 Mar 2025
HNWJKBGYW_n01_YZ88.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNWJKBGYW_n01_YZ88.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23956854
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC6173322.576849197311133TruSeq Adapter, Index 11 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATTC611450.25522967247702893TruSeq Adapter, Index 11 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTTTGC498580.20811580685844647TruSeq Adapter, Index 11 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGG455110.18997068646826498TruSeq Adapter, Index 11 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCGCGTATGC352360.14708108168125913TruSeq Adapter, Index 11 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATGTCGTATGC295650.12340935917545769TruSeq Adapter, Index 11 (98% over 50bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)