FastQCFastQC Report
Wed 12 Mar 2025
HNWJKBGYW_n01_YZ85.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNWJKBGYW_n01_YZ85.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12276735
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGGGGGTGG1009580.8223521970621668TruSeq Adapter, Index 8 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGGGGTTGG852150.6941177764283419TruSeq Adapter, Index 8 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGGGGTTTG739410.6022855425322775TruSeq Adapter, Index 8 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGGGGGTTG646440.526556938795209TruSeq Adapter, Index 8 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGGGGGGGG237570.19351236301834324TruSeq Adapter, Index 8 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGGGTTTTG177090.14424845042268974TruSeq Adapter, Index 8 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGGGTTTGG156680.12762350901929542TruSeq Adapter, Index 8 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGGGGATGG156080.1271347797276719TruSeq Adapter, Index 8 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGGGGGGTG147130.11984456779428733TruSeq Adapter, Index 8 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGGGGATTG144990.11810143332083001TruSeq Adapter, Index 8 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGGGTGTGG133100.10841644785849006TruSeq Adapter, Index 8 (97% over 42bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)