FastQCFastQC Report
Tue 16 Mar 2021
HNWF2AFX2_n01_AH7797-3h-anti-Hop1-SNP.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNWF2AFX2_n01_AH7797-3h-anti-Hop1-SNP.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17165907
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTAT8153804.7499966066459525TruSeq Adapter, Index 19 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATGTCGTAT217970.1269784346379134TruSeq Adapter, Index 19 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGAGCTCGTAT191360.11147677777818557TruSeq Adapter, Index 19 (97% over 40bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG177270.10326864755820941No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)