FastQCFastQC Report
Tue 16 Jul 2019
HNVWFBGXB_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNVWFBGXB_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73391124
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTCCTCAGCCTTGCGGGTTTCTTCGTCGGCCTTCTCCTTCTTTTCCT1750810.23855882081871374No Hit
CTCGAATTCTCCAGTTCCGCAAGCGACGACCAAGACGGCGCAATCGGCCT1692750.2306477824212094No Hit
GCCTCCTCCTCAGCCTTGCGGGTTTCTTCGTCGGCCTTCTCCTTCTTTTC1489890.20300683772059414No Hit
GTCCGTTCTTGGAGATTCCGGCCTCGAATTCTCCAGTTCCGCAAGCGACG1481940.201923600461549No Hit
GCCTCGGCCTCCTCAACAGAGTCGGTGATGATAATGTTGTCAAAGATCGT1276340.17390931361127537No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1243390.16941966987724563No Hit
CTTCAATTTGTCAAAAGTCTCGGCAGCATGGGCCTCGGCCTCCTCAACAG1202380.16383180069568085No Hit
CCGGCCTCGAATTCTCCAGTTCCGCAAGCGACGACCAAGACGGCGCAATC1110560.1513207509943573No Hit
CAGGAGTGTACTCTGGGTTCTCAATCTCTGGATGGATCCACTTGCCCTTG1017770.13867753272180436No Hit
GCCTCGAATTCTCCAGTTCCGCAAGCGACGACCAAGACGGCGCAATCGGC951730.12967916937748494No Hit
CCTGACTTGACCTGCCACAAATCGAATCCGATGGCTCCCCAGCTTTCATA868370.1183208476272962No Hit
GGCAGCATGGGCCTCGGCCTCCTCAACAGAGTCGGTGATGATAATGTTGT815140.11106792696075891No Hit
CTCGGCAGCATGGGCCTCGGCCTCCTCAACAGAGTCGGTGATGATAATGT796720.10855808666999023No Hit
CTTCTCAACAGTCTTCAATTTGTCAAAAGTCTCGGCAGCATGGGCCTCGG749620.10214041687111917No Hit
GGAATTTCAAATTTGGAACAAAGTAAAAGAATGAAAAAACTAGATATTAA748090.10193194479485013No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT3755550.067.42882512
ACGTCTG3745250.066.7558615
GCACACG3795800.066.62833411
CACGTCT3753850.066.453714
CGTCTGA3785600.065.92210416
CGGAAGA3935550.065.436954
ACACGTC3844600.065.32139613
GATCGGA3605400.065.201911
CTGAACT3880250.064.7760719
GTCTGAA3978900.063.53926517
CAGTCAC3513750.063.49157727
GAACTCC3935700.063.31912221
ACTCCAG3875850.063.022423
TGAACTC4001800.062.9518520
AACTCCA4019350.062.6628622
TCGGAAG4084550.062.2338263
GAGCACA4150200.062.0287069
CCAGTCA3732000.062.00378426
ATCGGAA4166450.061.252232
GGAAGAG4275050.060.39945
Produced by FastQC (version 0.11.5)