FastQCFastQC Report
Tue 16 Jul 2019
HNVWFBGXB_n01_AKM59.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNVWFBGXB_n01_AKM59.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34865816
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCGTATGC383570.11001320032205757TruSeq Adapter, Index 5 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG119750.058.278146
GTCACAC314850.049.49102829
GTATGCC149000.049.0455245
CACACAG316150.048.50142331
ATGCCGT127100.047.47308347
ACACAGT318900.046.9532532
CACAGTG323300.045.85966533
AGTCACA356300.044.85298528
CGTATGC163350.044.15846644
TCACACA355150.043.98365830
CAGTCAC379750.042.86655827
ACGTCTG434250.037.83992415
CCAGTCA437350.037.22088226
CGTCTGA448300.036.62271516
CACACGT455400.036.20020312
GTCTGAA462000.035.6875117
ACAGTGA418550.034.95507434
CTGAACT482000.034.26479319
CACGTCT483200.033.97038314
TCGTATG190850.033.92888343
Produced by FastQC (version 0.11.5)