FastQCFastQC Report
Tue 16 Jul 2019
HNVWFBGXB_n01_AKM58.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNVWFBGXB_n01_AKM58.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31127035
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC750890.241234026947957TruSeq Adapter, Index 3 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTAGG315050.062.19196732
CTTAGGC314700.061.08239733
CACTTAG327400.060.93565831
TCACTTA342300.059.6944430
TATGCCG136150.058.5335246
TTAGGCA334650.056.3213434
AGTCACT402950.051.9515728
GTATGCC165000.051.28995545
ATGCCGT141150.049.3931847
TAGGCAT371100.048.95043635
GTCACTT424500.048.6962229
CAGTCAC465650.047.4592627
AGGCATC325700.047.187336
CGTATGC186150.043.977344
ACGTCTG518450.043.02215215
CCAGTCA518500.042.56718426
CGTCTGA526100.042.4497216
CTGAACT534800.042.0267319
GTCTGAA536050.041.7460517
CACACGT537300.041.4565212
Produced by FastQC (version 0.11.5)