FastQCFastQC Report
Tue 16 Jul 2019
HNVWFBGXB_n01_AKM57.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNVWFBGXB_n01_AKM57.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19864656
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTAT16573528.343220240008185TruSeq Adapter, Index 19 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGCTCTCGTAT614920.30955481937366547TruSeq Adapter, Index 19 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGAGCTCGTAT319900.16103978845644243TruSeq Adapter, Index 19 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATATCGTAT258060.12990912100365593TruSeq Adapter, Index 19 (97% over 40bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATGCC257990.12987388253791055TruSeq Adapter, Index 19 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAATGATCTCGTAT213170.10731119632778942TruSeq Adapter, Index 19 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG2344700.066.8387948
ATGCCGT2347450.066.5600749
GTATGCC2366300.066.4574547
CACGTGA2369650.066.1209831
CGTATGC2399950.065.8746946
TGCCGTC2352150.065.7364850
CGTCTGA2609900.065.73559616
ACGTGAA2363500.065.7212632
ACGTCTG2613950.065.71023615
CACACGT2624850.065.7031612
TCACGTG2412850.065.6319730
GTCACGT2454600.065.6308929
GCCGTCT2305200.065.5386551
CAGTCAC2594250.065.5219827
GCACACG2634750.065.46110511
TCTCGTA2277950.065.4354143
CACGTCT2630550.065.37273414
CTCGTAT2323500.065.31354544
GTCTGAA2633200.065.3014817
CTGAACT2624900.065.2456319
Produced by FastQC (version 0.11.5)