FastQCFastQC Report
Tue 16 Jul 2019
HNVWFBGXB_n01_AKM56.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNVWFBGXB_n01_AKM56.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21143563
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTAT12782586.045613031256842TruSeq Adapter, Index 18 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCGCGTAT359870.17020310153023877TruSeq Adapter, Index 18 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACCTCTCGTAT350650.16584243630082593TruSeq Adapter, Index 18 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG1780400.067.2615548
ATGCCGT1773500.066.8191249
GTATGCC1813750.066.6868247
CCGCACA1749600.066.6649536
CGCACAT1749250.066.2113837
CGTATGC1825200.065.86594446
TCCGCAC1833950.065.74988635
TGCCGTC1755200.065.6661850
GCACATC1772050.065.3020338
GTCACGT2066150.065.2605129
GCCGTCT1654350.065.2113351
CACATCT1720900.065.1713739
CTCGTAT1813400.065.111944
TCTCGTA1811100.064.9477343
CGTCCGC1905250.064.9124333
CAGTCAC2160250.064.89519527
CGTCTGA2186550.064.83797516
TCACGTC2031850.064.8151230
TCGTATG1821150.064.7997545
GTCCGCA1888850.064.634934
Produced by FastQC (version 0.11.5)