FastQCFastQC Report
Tue 16 Jul 2019
HNVWFBGXB_n01_AKM55.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNVWFBGXB_n01_AKM55.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27318497
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTAT15262875.587009417099337TruSeq Adapter, Index 16 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGCTCTCGTAT949380.34752277916314356TruSeq Adapter, Index 16 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGAGCTCGTAT534830.19577577785483585TruSeq Adapter, Index 16 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCGCGTAT300980.1101744360240609TruSeq Adapter, Index 16 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATGTCGTAT277970.10175157147188588TruSeq Adapter, Index 16 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG2214650.068.0228848
CGTCCCG2124900.067.7081235
TCACCCG2226850.067.58295430
CCGTCCC2122200.067.5678234
ATGCCGT2216100.067.5489649
CCCGTCC2156650.067.4479133
GTATGCC2254650.067.4019247
CCCGATC1897500.067.3571538
CACCCGT2205200.067.3134431
GTCACCC2275400.067.3128329
GTCCCGA2025750.067.2359436
ACCCGTC2197000.066.924632
TGCCGTC2213350.066.3498250
CGTATGC2298000.066.3040646
AGTCACC2434000.066.2808228
TCCCGAT1993050.066.2778437
GCCGTCT2120150.065.9779751
CGTCTGA2610500.065.8333516
CTCGTAT2195000.065.8120244
ACGTCTG2616700.065.7604215
Produced by FastQC (version 0.11.5)