FastQCFastQC Report
Tue 16 Jul 2019
HNVWFBGXB_n01_AKM53.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNVWFBGXB_n01_AKM53.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19911213
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT8902734.471214285136722TruSeq Adapter, Index 14 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCGCGTAT1640160.8237368562126275TruSeq Adapter, Index 14 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGGAT610830.30677688998656183TruSeq Adapter, Index 14 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTTT606650.3046775703720311TruSeq Adapter, Index 14 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTGGTAT531900.26713590980117585TruSeq Adapter, Index 14 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCGCGGAT314730.15806671346441828TruSeq Adapter, Index 14 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCGGGTAT293140.147223576986495TruSeq Adapter, Index 14 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCGCGTTT257310.12922869139112722TruSeq Adapter, Index 14 (97% over 45bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG1055450.067.4644148
ATGCCGT1023950.066.6812849
TATCTCG1275600.066.6249541
GTCACAG2053800.066.56554429
AGTCACA2142800.066.55910528
GTATGCC1122450.066.5251547
CGTATCT1411850.066.354239
CGTCTGA2215700.066.1777416
ACGTCTG2217100.066.1643715
CAGTCAC2195150.066.0446927
CACACGT2230950.065.8961312
TCACAGT2033400.065.8317430
CACGTCT2231500.065.7750514
GCACACG2238450.065.7099111
GTCTGAA2236500.065.6306317
CCGTATC1856750.065.5743938
CTGAACT2244950.065.45219419
CACAGTT2014550.065.3476331
GTATCTC1302900.065.2692340
CGTATGC1080850.065.21924646
Produced by FastQC (version 0.11.5)