FastQCFastQC Report
Tue 16 Jul 2019
HNVWFBGXB_n01_AKM52.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNVWFBGXB_n01_AKM52.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21439092
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT18736508.739409299610264TruSeq Adapter, Index 13 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACACTCTCGTAT413430.19283932360568257TruSeq Adapter, Index 13 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATATCGTAT363440.16952210476078TruSeq Adapter, Index 13 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCGCGTAT318930.14876096431695893TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG2345100.067.5664148
GTATGCC2382600.067.3377847
ATGCCGT2334300.067.1942649
GTCACAG2642250.066.9839129
ACGTCTG2803250.066.85396615
AGTCACA2733100.066.845428
CACAGTC2565700.066.7877831
CGTATGC2403800.066.67142546
TGCCGTC2291150.066.61465550
CGTCTGA2811250.066.5739416
CACACGT2827400.066.4395312
CTCGTAT2375400.066.4252244
TCACAGT2632100.066.3713730
CACGTCT2829300.066.3486814
TCGTATG2402000.066.2912445
CAGTCAC2794400.066.2741127
GCCGTCT2173350.066.2168251
GTCTGAA2833400.066.16991417
AGTCAAC2514750.066.1094934
CTGAACT2839200.066.06077619
Produced by FastQC (version 0.11.5)