FastQCFastQC Report
Tue 16 Jul 2019
HNVWFBGXB_n01_AKM51.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNVWFBGXB_n01_AKM51.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19535046
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC9938895.0877228546070485TruSeq Adapter, Index 12 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGTCGTATGC2908831.4890315589735494TruSeq Adapter, Index 12 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATTC730930.3741634393899047TruSeq Adapter, Index 12 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGG504660.25833571111119985TruSeq Adapter, Index 12 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCGCGTATGC402560.20607066909389413TruSeq Adapter, Index 12 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTTTGC364980.1868334479478574TruSeq Adapter, Index 12 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGTCGTATTC321520.16458625180611297TruSeq Adapter, Index 12 (97% over 48bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGTCGTATGG299110.15311456138879836TruSeq Adapter, Index 12 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGGATGC299060.15308896636332467TruSeq Adapter, Index 12 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGGCGTATGC247080.1264803778808609TruSeq Adapter, Index 12 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGTCGTTTGC220680.11296620443074462TruSeq Adapter, Index 12 (97% over 46bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG1588100.068.3122346
GTATGCC1667100.067.9995445
GTCACCT2588050.067.616629
AGTCACC2645800.067.6090528
ATGCCGT1460000.067.4715347
CGTATGC1664900.067.37675544
CAGTCAC2750850.067.24721527
ACGTCTG2797650.067.1187215
CACCTTG2525950.067.0033931
ACCTTGT2492350.066.8581732
CGTCTGA2805750.066.8461916
TCGTATG1696500.066.8210943
CACACGT2820500.066.7999412
TCACCTT2574300.066.7852130
CACGTCT2820850.066.63476614
CCAGTCA2769000.066.5762126
GTCTGAA2823550.066.57426517
TGTAATC1750250.066.534536
CTGAACT2821950.066.4878119
CCTTGTA2497750.066.4323133
Produced by FastQC (version 0.11.5)