FastQCFastQC Report
Tue 16 Jul 2019
HNVWFBGXB_n01_AKM50.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNVWFBGXB_n01_AKM50.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18992261
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC5718093.0107473775765823TruSeq Adapter, Index 11 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG268450.14134704656807318No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG750000.067.6374746
GTATGCC766650.067.1725345
GGCTACA770950.066.8602434
GCTACAT755950.066.7848835
ACGGCTA805800.066.7391332
CGGCTAC800750.066.2335533
CACGGCT822650.066.0144131
ATGCCGT749350.065.7808347
CTACATC744600.065.3538236
ACGTCTG924000.065.3531815
TCACGGC842550.065.332130
CGTATGC789350.065.258744
CAGTCAC922350.064.82448627
TGCCGTC731450.064.7488448
CACACGT936400.064.6491712
CTCGTAT785200.064.60647642
TCGTATG803600.064.52904543
CGTCTGA939400.064.2891116
GCCGTCT709000.064.21886449
AGTCACG911300.063.9780528
Produced by FastQC (version 0.11.5)