FastQCFastQC Report
Tue 16 Jul 2019
HNVWFBGXB_n01_AKM49.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNVWFBGXB_n01_AKM49.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24432706
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC1815430.7430327201579718TruSeq Adapter, Index 10 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGCGTATGC947530.3878121400061049TruSeq Adapter, Index 10 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATTC462860.18944279033194278TruSeq Adapter, Index 10 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGCGGATGC394900.161627615050089TruSeq Adapter, Index 10 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGG368000.1506177825738991TruSeq Adapter, Index 10 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGGATGC362560.1483912588315023TruSeq Adapter, Index 10 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGCGTATGG321580.1316186590220502TruSeq Adapter, Index 10 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTTTGC267670.10955397244987927TruSeq Adapter, Index 10 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGCGTATTC260110.106459759307872TruSeq Adapter, Index 10 (97% over 48bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCTTA1634850.067.4570934
ACTAGCT1679750.066.9876732
CACTAGC1718900.066.7354231
CTAGCTT1666350.066.4723233
TCACTAG1754350.066.45441430
GTCACTA1780600.066.3928329
AGTCACT1810350.065.9239228
AGCTTAT1646050.065.6547635
CAGTCAC1858650.065.566527
ACGTCTG1885550.065.20705415
CGTCTGA1899700.064.6788416
GCTTATC1586050.064.631436
CACACGT1907750.064.573312
CACGTCT1909450.064.4623614
CTTATCT801300.064.4325937
TATGCCG368700.064.3599146
CTGAACT1917350.064.1642819
TATCGCG491300.064.1631839
GTATGCC409850.064.0890445
ACACGTC1932300.063.85402313
Produced by FastQC (version 0.11.5)