FastQCFastQC Report
Tue 16 Jul 2019
HNVWFBGXB_n01_AKM48.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNVWFBGXB_n01_AKM48.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19402287
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC16024098.258866596499681TruSeq Adapter, Index 9 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATATCGTATGC644280.33206394689450786TruSeq Adapter, Index 9 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGCTCTCGTATGC377270.19444614956989348TruSeq Adapter, Index 9 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGAGCTCGTATGC288430.14865773297756085TruSeq Adapter, Index 9 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATGTCGTATGC223330.11510498736566467TruSeq Adapter, Index 9 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG2080950.068.56246
GTATGCC2090300.068.4023145
ATGCCGT2076100.068.1403847
CTCGTAT1997950.067.601942
TGCCGTC2078700.067.5887748
CGTATGC2126300.067.5887444
ACGTCTG2271600.067.4143315
TCGTATG2143850.067.4139943
GCCGTCT2056750.067.3745549
CAGTCAC2256150.067.329127
CACACGT2286550.067.1178612
CAGATCT1875900.067.1159337
CGTCTGA2282350.067.03393616
CACGTCT2288550.066.98402414
GTCACGA2189250.066.9333929
TCTCGTA1970300.066.9044841
GCACACG2296500.066.7965811
ACACGTC2307150.066.613913
AGTCACG2242550.066.5578528
GTCTGAA2304050.066.50098417
Produced by FastQC (version 0.11.5)