FastQCFastQC Report
Tue 16 Jul 2019
HNVWFBGXB_n01_AKM47.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNVWFBGXB_n01_AKM47.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21566225
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC1018990.4724934475087782TruSeq Adapter, Index 8 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGCGTATGC575400.2668060821956555TruSeq Adapter, Index 8 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGCGGATGC336040.1558177196055406TruSeq Adapter, Index 8 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATTC317820.14736932402402367TruSeq Adapter, Index 8 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGGATGC297200.1378080772133278TruSeq Adapter, Index 8 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGG276150.128047444557404TruSeq Adapter, Index 8 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGCGTATGG244200.1132326125689591TruSeq Adapter, Index 8 (97% over 49bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG238500.11058959089965906No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGCGGATGG234430.10870238069017643TruSeq Adapter, Index 8 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGGGGGTGG223150.10347197991303532TruSeq Adapter, Index 8 (97% over 42bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACAC1555750.066.7840229
AGTCACA1616500.066.23340628
CAGTCAC1651550.066.0520727
ACGTCTG1670650.066.042815
CGTCTGA1679850.065.5830416
TCACACT1570550.065.4012830
CACGTCT1689250.065.39242614
ACACTTG1528750.065.3464932
CACACGT1695550.065.2674212
GCACACG1701650.064.9923211
CTGAACT1695950.064.9501319
GTCTGAA1706700.064.6022417
CACACTT1569150.064.5452631
ACACGTC1718150.064.4944513
ACTCCAG1698050.064.3136423
TCTGAAC1718200.064.1861518
CACTTGA1541350.064.07686633
AGCACAC1734550.063.92765810
TCCAGTC1703850.063.88813825
CCAGTCA1705100.063.82707226
Produced by FastQC (version 0.11.5)