FastQCFastQC Report
Tue 16 Jul 2019
HNVWFBGXB_n01_AKM46.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNVWFBGXB_n01_AKM46.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20843444
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC2641341.2672281989483118TruSeq Adapter, Index 7 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCGCGTATGC848100.40689053114255014TruSeq Adapter, Index 7 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGG552280.26496580891334465TruSeq Adapter, Index 7 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGGATGC406320.19493899376705692TruSeq Adapter, Index 7 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTTTGC401940.1928376135920724TruSeq Adapter, Index 7 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATTC359810.17262502300483548TruSeq Adapter, Index 7 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCGCGTATGG308020.1477778816207149TruSeq Adapter, Index 7 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCGCGGATGC274900.13188799317425662TruSeq Adapter, Index 7 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCGCGTTTGC209360.100444053295607TruSeq Adapter, Index 7 (97% over 46bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG1963900.066.9595315
AGTCACC1891050.066.8652428
CACACGT1978850.066.62005612
CAGTCAC1956150.066.4559227
CACGTCT1980150.066.4469914
CGTCTGA1979150.066.41498616
GCACACG1990050.066.2486311
TCACCAG1858550.066.1671630
ACACGTC2001150.065.9126713
GTCACCA1893650.065.8559429
CTGAACT1997600.065.7498119
ACCAGAT1812500.065.53947432
GTCTGAA2011300.065.5017417
TCTGAAC2012900.065.2879918
CAGATCA1735550.065.2626534
AGCACAC2025750.065.2127510
ACTCCAG2002650.064.8045423
TCCAGTC2005550.064.67193625
CCAGATC1796900.064.5677133
CCAGTCA2003950.064.5489126
Produced by FastQC (version 0.11.5)