FastQCFastQC Report
Tue 16 Jul 2019
HNVWFBGXB_n01_AKM45.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNVWFBGXB_n01_AKM45.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21330449
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC10007664.691724960876352TruSeq Adapter, Index 6 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG1221700.067.9747446
GTATGCC1246450.067.787345
GTCACGC1393150.067.1248829
CACGCCA1356350.067.0644231
TCACGCC1365850.067.0262230
ATGCCGT1201700.066.8138247
CGTATGC1267400.066.5730544
TATCTCG1312500.066.486939
ACGTCTG1516250.066.4696415
CAGTCAC1500850.066.1856427
GCCAATA1339150.066.1566834
ACGCCAA1369700.066.0712232
CTCGTAT1290700.066.06441542
TCGTATG1292050.066.0631243
CACACGT1527900.066.0033712
CGCCAAT1351400.065.9587533
TGCCGTC1164750.065.946348
CGTCTGA1528000.065.91025516
CACGTCT1531600.065.8697414
GCCGTCT1123450.065.74563649
Produced by FastQC (version 0.11.5)