FastQCFastQC Report
Tue 16 Jul 2019
HNVWFBGXB_n01_AKM44.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNVWFBGXB_n01_AKM44.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31670450
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC14580524.603824700943624TruSeq Adapter, Index 4 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATATCGTATGC829680.2619729116573967TruSeq Adapter, Index 4 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG2039250.066.9684946
GTATGCC2079550.066.6736645
ATGCCGT2007050.066.21889547
ACTGACC2124200.066.0562232
CGTATGC2119000.065.6589744
AGTCACT2366350.065.3501228
TGCCGTC1969750.065.28902448
CTGACCA2132950.065.1142833
TCGTATG2164750.064.96268543
ACGTCTG2465450.064.8953415
GCCGTCT1910300.064.8223949
CAGTCAC2443400.064.8060227
CTCGTAT2007050.064.7844542
CACTGAC2217450.064.7439331
CACACGT2478150.064.5817812
ACCAATC1871050.064.56303436
GTCACTG2347400.064.4813129
GACCAAT2047050.064.44835
CGTCTGA2484100.064.3037616
CACGTCT2491700.064.2454814
Produced by FastQC (version 0.11.5)