FastQCFastQC Report
Tue 16 Jul 2019
HNVWFBGXB_n01_AKM43.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNVWFBGXB_n01_AKM43.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23002190
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC5589992.430199037569901TruSeq Adapter, Index 2 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGCGTATGC1564730.6802526194244982TruSeq Adapter, Index 2 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATTC811150.35264033555065843TruSeq Adapter, Index 2 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGG679180.29526753756924884TruSeq Adapter, Index 2 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGGATGC622940.2708176917067462TruSeq Adapter, Index 2 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTTTGC488780.21249281046717725TruSeq Adapter, Index 2 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGCGGATGC402870.17514419279207763TruSeq Adapter, Index 2 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGCGTATGG356620.15503741165515111TruSeq Adapter, Index 2 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTGGTATGC295580.12850080796654578TruSeq Adapter, Index 2 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGCGTATTC271390.11798441800541601TruSeq Adapter, Index 2 (97% over 48bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGCGTTTGC241100.10481610664028077TruSeq Adapter, Index 2 (97% over 46bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG863150.067.90221446
GTATGCC931150.067.3187545
GTCACCG2269300.067.00185429
AGTCACC2322950.066.90739428
ATGTATC2043250.066.8124736
TATCTCG1221750.066.7244839
ACCGATG2202550.066.6821132
CGATGTA2160050.066.52602434
CCGATGT2176350.066.46200633
ATGCCGT738000.066.4606947
ACGTCTG2421400.066.38734415
CACCGAT2245050.066.34798431
TCACCGA2279000.066.246230
GTATCTC1258700.066.2405138
TGTATCT1403950.066.22410637
CAGTCAC2409600.066.07114427
CGTCTGA2433750.066.00300616
CACACGT2442000.065.9038512
GTATCGC522350.065.8003238
CACGTCT2447950.065.7157814
Produced by FastQC (version 0.11.5)