FastQCFastQC Report
Tue 16 Jul 2019
HNVWFBGXB_n01_AKM42.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNVWFBGXB_n01_AKM42.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26909843
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC19201887.135634347625142TruSeq Adapter, Index 1 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATATCGTATGC712030.2645983478982022TruSeq Adapter, Index 1 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATGTCGTATGC556910.20695401307246572TruSeq Adapter, Index 1 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGAGCTCGTATGC549210.20409260656035788TruSeq Adapter, Index 1 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGCTCTCGTATGC542310.20152848903652096TruSeq Adapter, Index 1 (98% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG272240.10116744270860295No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG2601650.068.1045546
GTATGCC2623950.067.87630545
ATGCCGT2593750.067.48849547
CGTATGC2659150.067.2727644
TCGTATG2689050.066.9784543
GCCGTCT2517400.066.918949
CTCGTAT2492150.066.8794242
TGCCGTC2574400.066.853448
AGTCACA2871750.066.465728
GTCACAT2834400.066.3788729
CAGTCAC2933900.066.3218927
TCTCGTA2439700.066.2707841
ACATCAC2712200.065.9725232
CCGTCTT2572200.065.6803850
CATCACG2684700.065.53255533
ACGTCTG3002250.065.4866315
TCACATC2811750.065.2805430
CCAGTCA2972700.065.2028726
CACATCA2772450.065.1984831
CTGAACT3019900.065.1184819
Produced by FastQC (version 0.11.5)