FastQCFastQC Report
Tue 16 Jul 2019
HNVWFBGXB_n01_AKM41.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNVWFBGXB_n01_AKM41.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37731320
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCGGGGGT1103940.2925792153574272TruSeq Adapter, Index 21 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCGGGGTT682080.1807728963630215TruSeq Adapter, Index 21 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCGCGGAT520950.1380683209598816TruSeq Adapter, Index 21 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTAT518570.13743754525418142TruSeq Adapter, Index 21 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCGCGGTT508330.13472361952881584TruSeq Adapter, Index 21 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCGCGGGT493740.13085680543378816TruSeq Adapter, Index 21 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGGAT442350.11723682076322801TruSeq Adapter, Index 21 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG1362550.065.20711528
GTCACGT1328800.064.701529
CAGTCAC1442150.063.39898727
CCAGTCA1442550.063.25975426
CGTTTCG1283150.062.81740633
ACTCCAG1461800.062.7414323
TCCAGTC1458400.062.6199125
ACGTCTG1484900.062.44775415
CGGAATC1203850.062.1997238
GTTTCGG1287550.062.1297334
GAACTCC1480500.062.0801821
CGTCTGA1498000.061.84308216
CACGTCT1501950.061.78787614
ACACGTC1507550.061.70717613
GCACACG1514100.061.2911711
TCACGTT1371000.061.14048430
CACACGT1521300.061.0790712
CTCCAGT1499050.061.07094224
ACGTTTC1357350.060.29099732
CTGAACT1558700.059.52215619
Produced by FastQC (version 0.11.5)