FastQCFastQC Report
Tue 16 Jul 2019
HNVWFBGXB_n01_AKM40.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNVWFBGXB_n01_AKM40.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19147033
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTAT13647067.127506387020903TruSeq Adapter, Index 20 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCGCGTAT1326370.6927287376587276TruSeq Adapter, Index 20 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTTT580250.30304956386715376TruSeq Adapter, Index 20 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGGAT358420.18719349363423565TruSeq Adapter, Index 20 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTGGTAT266630.13925395125187282TruSeq Adapter, Index 20 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCTTA2115900.068.43162536
ACGTCTG2534900.068.03841415
AGTCACG2423050.067.9903728
GCACACG2542700.067.9723111
ACACGTC2544400.067.93706513
CACGTCT2541900.067.8827214
GCCTTAT2124900.067.8795537
CACACGT2548450.067.86684412
GAACTCC2523500.067.8322721
GTCACGT2379800.067.7870929
CGTCTGA2542900.067.78445416
ACTCCAG2518150.067.77521523
CGTGGCC2188450.067.7381933
TCCAGTC2510750.067.63676525
GTATGCC1702300.067.5496447
CAGTCAC2514200.067.5439727
CCAGTCA2511100.067.5329126
TGGCCTT2148750.067.4750435
GATCGGA2487550.067.4740451
TCGGAAG2544700.067.4673
Produced by FastQC (version 0.11.5)