FastQCFastQC Report
Sat 15 Mar 2025
HNVHVAFX7_n02_dam126-GR-bZIP1-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNVHVAFX7_n02_dam126-GR-bZIP1-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12812506
Sequences flagged as poor quality0
Sequence length76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATAC12107829.450001428292014Clontech Universal Primer Mix Long (95% over 23bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAACTAATAC4447383.4711242281564587Clontech Universal Primer Mix Long (95% over 23bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGCTAATAC3878033.026753704544607Clontech Universal Primer Mix Long (95% over 23bp)
GGTCGCGGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCGCG2486921.9410098227466195Clontech Universal Primer Mix Long (95% over 23bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGACCTAATAC2042821.5943953509172992Clontech Universal Primer Mix Long (95% over 23bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGACTAATACG1314881.0262473242939358Clontech Universal Primer Mix Long (95% over 23bp)
CTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCT941380.7347352656849487Clontech Universal Primer Mix Long (95% over 24bp)
CTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCC897830.7007450376998848Clontech Universal Primer Mix Long (100% over 23bp)
ATAGTGAGTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCTATAGTGA724190.5652211987256825No Hit
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATCCTAATA719480.5615451028861957Clontech Universal Primer Mix Long (95% over 23bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGG694710.5422124290127162Clontech Universal Primer Mix Long (100% over 23bp)
AGTGAGTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCTATAGTGAGT597330.46620856216574647No Hit
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGC554770.43299101674567025Clontech Universal Primer Mix Long (95% over 24bp)
CAGCGTGGTCGCGGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTG539980.42144760751721794Clontech Universal Primer Mix Long (95% over 23bp)
CTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGACTCACTA431980.33715496406401685No Hit
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATTCTAATA407850.31832180215174144Clontech Universal Primer Mix Long (95% over 23bp)
GTGAGTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTC407520.31806424129674554No Hit
GTCGCGGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCGCGG382000.29814620184372986Clontech Universal Primer Mix Long (95% over 23bp)
CGGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGA380800.2972096169164721Clontech Universal Primer Mix Long (95% over 23bp)
AATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGA363380.28361352572244647Clontech Universal Primer Mix Long (95% over 21bp)
CCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGACCACGCT354840.27694816299012853Clontech Universal Primer Mix Long (95% over 24bp)
CGTGGTCGCGGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTC345370.26955694693918586Clontech Universal Primer Mix Long (95% over 23bp)
CGTATTAGTCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAG327280.2554379291607746Clontech Universal Primer Mix Long (95% over 24bp)
CGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGACTCA296560.2314613550229752No Hit
CTGCCCTATAGTGAGTCGTATTAGTTCCTCGGCCGCGACCACGCTGCCCT295160.2303686726078411Clontech Universal Primer Mix Long (95% over 24bp)
CTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGTTCCTCGGCC278490.21735794699335168Clontech Universal Primer Mix Long (100% over 23bp)
CTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGCTCCTCGGCC275220.21480575306657418Clontech Universal Primer Mix Long (100% over 23bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATTAATACG269360.21023209667179865Clontech Universal Primer Mix Long (95% over 23bp)
ATCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCG264850.2067120983201881Clontech Universal Primer Mix Long (95% over 24bp)
CGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGACC248670.19408381155099555Clontech Universal Primer Mix Long (100% over 23bp)
CAGCGTGGTCGCGGCCGAGGAACTAATACGACTCACTATAGGGCAGCGTG239280.18675503449520336Clontech Universal Primer Mix Long (95% over 23bp)
CGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGACCAC237940.18570918132643216Clontech Universal Primer Mix Long (100% over 23bp)
TAGTGAGTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCTATAGTGAG235280.1836330847376774No Hit
CGCGGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCC233040.18188479287346285Clontech Universal Primer Mix Long (95% over 23bp)
CTGCCCTATAGTGAGTCGTATTAGCTCCTCGGCCGCGACCACGCTGCCCT231660.1808077202071164Clontech Universal Primer Mix Long (95% over 24bp)
GTATTAGATCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAG228960.17870040412078636Clontech Universal Primer Mix Long (100% over 23bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGTTCCTCGG221850.17315113842678395Clontech Universal Primer Mix Long (100% over 23bp)
CTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGACTCACTATAGG221320.17273748008391177No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGCTCCTCGG212780.16607211735159383Clontech Universal Primer Mix Long (100% over 23bp)
CGTGGTCGCGGCCGAGGAACTAATACGACTCACTATAGGGCAGCGTGGTC210080.16396480126526378Clontech Universal Primer Mix Long (95% over 23bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGA203340.15870431592383255Clontech Universal Primer Mix Long (95% over 23bp)
CGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAT200370.15638626822886953Clontech Universal Primer Mix Long (95% over 23bp)
CGTATTAGATCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTA195750.15278041625892702Clontech Universal Primer Mix Long (100% over 22bp)
ATAGTGAGTCGTATTAGTTCCTCGGCCGCGACCACGCTGCCCTATAGTGA191950.14981456398927734No Hit
CTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAACTAATACGACTCACTA191540.14949456413913093No Hit
CAGCGTGGTCGCGGCCGAGGAGCTAATACGACTCACTATAGGGCAGCGTG184690.14414822517936773Clontech Universal Primer Mix Long (95% over 23bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAATACGACT174900.13650725314782292Clontech Universal Primer Mix Long (95% over 23bp)
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGTTCCTCGGC172590.1347043271628517Clontech Universal Primer Mix Long (95% over 24bp)
CCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCTATA171530.1338770104771073Clontech Universal Primer Mix Short (100% over 21bp)
CGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGC167620.13082530458912567Clontech Universal Primer Mix Long (100% over 23bp)
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGCTCCTCGGC166630.13005262202413798Clontech Universal Primer Mix Long (95% over 24bp)
CACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGACCACGCTG166020.12957652468611527Clontech Universal Primer Mix Long (100% over 23bp)
ATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGAC164400.12831213503431727No Hit
CGTGGTCGCGGCCGAGGAGCTAATACGACTCACTATAGGGCAGCGTGGTC162590.12689945276903675Clontech Universal Primer Mix Long (95% over 23bp)
GGTCGCGGCCGAGGATCCTAATACGACTCACTATAGGGCAGCGTGGTCGC152770.1192350661143105Clontech Universal Primer Mix Long (95% over 23bp)
CCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGAC148240.11569945801391235Clontech Universal Primer Mix Long (95% over 24bp)
ATAGTGAGTCGTATTAGCTCCTCGGCCGCGACCACGCTGCCCTATAGTGA147940.1154653117820979No Hit
GCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGG147190.11487994620256177Clontech Universal Primer Mix Long (95% over 23bp)
CTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGGTCCTCGGCC146550.11438043424135762Clontech Universal Primer Mix Long (100% over 23bp)
CTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGCTAATACGACTCACTA146360.11423214162787515No Hit
CACTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGACTCACTATA144790.1130067763480462No Hit
ATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGACTCACTATAGGGC144480.11276482524183794Clontech Universal Primer Mix Short (100% over 22bp)
CCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGA140320.10951799749401092Clontech Universal Primer Mix Long (95% over 23bp)
AATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAACTAATACGA138350.1079804372384294Clontech Universal Primer Mix Long (95% over 21bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAA137630.1074184862820747Clontech Universal Primer Mix Long (95% over 23bp)
ACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGACTCACT137300.10716092542707883No Hit
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGACTCTAATA136520.10655214522436127Clontech Universal Primer Mix Long (95% over 23bp)
CTGCCCTATAGTGAGTCGTATTAGGTCCTCGGCCGCGACCACGCTGCCCT135250.10556092617634676Clontech Universal Primer Mix Long (95% over 24bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGAGATCGGAAG133140.10391409767925182Clontech Universal Primer Mix Long (95% over 23bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)