FastQCFastQC Report
Sat 15 Mar 2025
HNVHVAFX7_n02_dam126-GR-EV-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNVHVAFX7_n02_dam126-GR-EV-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13945891
Sequences flagged as poor quality0
Sequence length76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATAC9194846.593225201602393Clontech Universal Primer Mix Long (95% over 23bp)
GGTCGCGGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCGCG4294193.0791793797900757Clontech Universal Primer Mix Long (95% over 23bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAACTAATAC1905021.3660080951442972Clontech Universal Primer Mix Long (95% over 23bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGACCTAATAC1370870.9829920512070545Clontech Universal Primer Mix Long (95% over 23bp)
CTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCT1188500.852222349938057Clontech Universal Primer Mix Long (95% over 24bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGCTAATAC1149960.8245869697389718Clontech Universal Primer Mix Long (95% over 23bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGG1110150.7960409270372183Clontech Universal Primer Mix Long (100% over 23bp)
CTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCC827960.5936945871726661Clontech Universal Primer Mix Long (100% over 23bp)
CGGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGA776480.5567804882456058Clontech Universal Primer Mix Long (95% over 23bp)
GTCGCGGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCGCGG669680.4801987911708187Clontech Universal Primer Mix Long (95% over 23bp)
CAGCGTGGTCGCGGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTG603820.43297341130803335Clontech Universal Primer Mix Long (95% over 23bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATCCTAATA576450.4133475587898974Clontech Universal Primer Mix Long (95% over 23bp)
CCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGACCACGCT544760.3906240196485115Clontech Universal Primer Mix Long (95% over 24bp)
CGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGACC509080.36503942272315193Clontech Universal Primer Mix Long (100% over 23bp)
CGTGGTCGCGGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTC475840.3412044450942575Clontech Universal Primer Mix Long (95% over 23bp)
CGCGGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCC467690.3353604298212283Clontech Universal Primer Mix Long (95% over 23bp)
CGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGACCAC466140.3342489913337197Clontech Universal Primer Mix Long (100% over 23bp)
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGC462250.33145963925861743Clontech Universal Primer Mix Long (95% over 24bp)
AATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGA460840.33044858876352895Clontech Universal Primer Mix Long (95% over 21bp)
GTATTAGATCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAG433200.3106291308314399Clontech Universal Primer Mix Long (100% over 23bp)
CTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGACTCACTA432660.3102419200035337No Hit
CGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGACTCA403020.28898834789401406No Hit
GCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGG393290.2820113824208148Clontech Universal Primer Mix Long (95% over 23bp)
CGTATTAGATCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTA338770.2429174299440602Clontech Universal Primer Mix Long (100% over 22bp)
GTGAGTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTC329220.2360695347468297No Hit
ATAGTGAGTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCTATAGTGA327420.2347788319871423No Hit
ATCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCG325890.23368173464140798Clontech Universal Primer Mix Long (95% over 24bp)
GGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAG319950.2294224155344395Clontech Universal Primer Mix Long (95% over 23bp)
CGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGC319750.22927900411669647Clontech Universal Primer Mix Long (100% over 23bp)
CCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGA308570.22126230586486012Clontech Universal Primer Mix Long (95% over 23bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGACTAATACG305560.21910396402782728Clontech Universal Primer Mix Long (95% over 23bp)
CCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGAC302530.2169312810490201Clontech Universal Primer Mix Long (95% over 24bp)
CGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAT301870.21645802337046804Clontech Universal Primer Mix Long (95% over 23bp)
CACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGACCACGCTG285440.20467677540287674Clontech Universal Primer Mix Long (100% over 23bp)
CCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCTATA274760.19701860569539803Clontech Universal Primer Mix Short (100% over 21bp)
CTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGACTCACTATAGG273430.19606491976740678No Hit
GTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTAT260030.18645635477862263No Hit
ACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGACTCACT244790.17552840474660242No Hit
GGTCGCGGCCGAGGATCCTAATACGACTCACTATAGGGCAGCGTGGTCGC238190.17079582796108186Clontech Universal Primer Mix Long (95% over 23bp)
ATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGAC237890.1705807108344673No Hit
GGCAGCGTGGTCGCGGCCGAGGATCTAATACGACTCACTATAGGGCAGCG236700.16972741289889617Clontech Universal Primer Mix Long (95% over 23bp)
TCGCGGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCGCGGC235380.16878089754179204Clontech Universal Primer Mix Long (95% over 23bp)
TAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACG227800.16334560480933058Clontech Universal Primer Mix Long (95% over 22bp)
GGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCG226640.1625138185864209Clontech Universal Primer Mix Long (95% over 24bp)
GTGGTCGCGGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCG223160.1600184599176919Clontech Universal Primer Mix Long (95% over 23bp)
ATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGACTCACTATAGGGC217740.15613201049685532Clontech Universal Primer Mix Short (100% over 22bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATTCTAATA216660.15535758884104287Clontech Universal Primer Mix Long (95% over 23bp)
CACTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGACTCACTATA208390.14942752671736786No Hit
AGCGTGGTCGCGGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGG199400.1429811834898179Clontech Universal Primer Mix Long (95% over 23bp)
GCGGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCG187850.13469917411515692Clontech Universal Primer Mix Long (95% over 23bp)
TAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGACTCACTATAGGGCA180140.12917066396116247Clontech Universal Primer Mix Long (100% over 23bp)
GCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGA177360.1271772452545341Clontech Universal Primer Mix Long (100% over 23bp)
CCTATAGTGAGTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCTATAG173550.12444525774652906No Hit
AGTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTA169450.12150532368279661No Hit
AGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGACTCACTATAGGGCAG168590.1208886545865015Clontech Universal Primer Mix Long (95% over 23bp)
CTGCCCTATAGTGAGTCGTATTAGTTCCTCGGCCGCGACCACGCTGCCCT167710.1202576443484321Clontech Universal Primer Mix Long (95% over 24bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGGTCCTCGG165540.11870163046592004Clontech Universal Primer Mix Long (100% over 23bp)
CTGCCCTATAGTGAGTCGTATTAGGTCCTCGGCCGCGACCACGCTGCCCT163540.11726751628848957Clontech Universal Primer Mix Long (95% over 24bp)
ACTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGACTCACTATAG160840.11533146214895842No Hit
CTATAGTGAGTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCTATAGT160330.11496576303371366No Hit
CGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGACCACGCTGCC156260.11204734068264266Clontech Universal Primer Mix Long (95% over 24bp)
GAGTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGT152870.109616517151898No Hit
ATTAGATCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAT151210.10842620238463072Clontech Universal Primer Mix Long (100% over 23bp)
CGTGGTCGCGGCCGAGGAACTAATACGACTCACTATAGGGCAGCGTGGTC149640.1073004227553478Clontech Universal Primer Mix Long (95% over 23bp)
TGGTCGCGGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCGC147260.10559382688420553Clontech Universal Primer Mix Long (95% over 23bp)
AGTGAGTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCTATAGTGAGT145750.10451107068024552No Hit
GACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGACCACG144120.1033422676256397Clontech Universal Primer Mix Long (95% over 24bp)
CGTATTAGTCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAG143640.10299808022305638Clontech Universal Primer Mix Long (95% over 24bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGTTCCTCGG140930.10105485551263808Clontech Universal Primer Mix Long (100% over 23bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)