FastQCFastQC Report
Sat 15 Mar 2025
HNVHVAFX7_n02_dam126-GR-EV-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNVHVAFX7_n02_dam126-GR-EV-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17453960
Sequences flagged as poor quality0
Sequence length76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATAC14842628.5038696089598Clontech Universal Primer Mix Long (95% over 23bp)
GGTCGCGGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCGCG3633682.081865662577432Clontech Universal Primer Mix Long (95% over 23bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAACTAATAC2914031.6695523537351982Clontech Universal Primer Mix Long (95% over 23bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGACCTAATAC2744041.5721589828325493Clontech Universal Primer Mix Long (95% over 23bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGCTAATAC2193581.2567806961858512Clontech Universal Primer Mix Long (95% over 23bp)
CTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCT1105700.6334952068183953Clontech Universal Primer Mix Long (95% over 24bp)
CTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCC1097120.6285794169346097Clontech Universal Primer Mix Long (100% over 23bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGACTAATACG1058710.6065729496343524Clontech Universal Primer Mix Long (95% over 23bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATCCTAATA997680.5716066726404782Clontech Universal Primer Mix Long (95% over 23bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGCTAATA988320.5662439927672575Clontech Universal Primer Mix Long (95% over 23bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGG950780.5447359796859853Clontech Universal Primer Mix Long (100% over 23bp)
ATAGTGAGTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCTATAGTGA842490.4826927528194175No Hit
AGTGAGTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCTATAGTGAGT775750.4444550119285251No Hit
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGC731280.41897655317188764Clontech Universal Primer Mix Long (95% over 24bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGGCTAATA692500.3967580995945906Clontech Universal Primer Mix Long (95% over 23bp)
CAGCGTGGTCGCGGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTG668540.38303055581655965Clontech Universal Primer Mix Long (95% over 23bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATTCTAATA625630.3584458770387924Clontech Universal Primer Mix Long (95% over 23bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGACTCTAATA611700.35046488017618926Clontech Universal Primer Mix Long (95% over 23bp)
GTCGCGGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCGCGG570890.3270833667545932Clontech Universal Primer Mix Long (95% over 23bp)
CTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGACTCACTA549480.3148168094804846No Hit
CGGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGA528550.3028252614306438Clontech Universal Primer Mix Long (95% over 23bp)
GTGAGTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTC500720.28688045578195437No Hit
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATGCTAATA480760.2754446555394879Clontech Universal Primer Mix Long (95% over 23bp)
AATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGA476670.2731013477743733Clontech Universal Primer Mix Long (95% over 21bp)
CGTGGTCGCGGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTC473700.27139972819921665Clontech Universal Primer Mix Long (95% over 23bp)
CCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGACCACGCT438530.2512495731627665Clontech Universal Primer Mix Long (95% over 24bp)
CGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGACTCA413800.23708086875413947No Hit
ATCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCG366780.21014142349357967Clontech Universal Primer Mix Long (95% over 24bp)
GTATTAGATCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAG364510.20884085903714686Clontech Universal Primer Mix Long (100% over 23bp)
CGCGGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCC356590.20430320683672934Clontech Universal Primer Mix Long (95% over 23bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGA328550.18823808465242273Clontech Universal Primer Mix Long (95% over 23bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAATACGACT328140.18800318094002738Clontech Universal Primer Mix Long (95% over 23bp)
CGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGACC312200.17887058295080316Clontech Universal Primer Mix Long (100% over 23bp)
CGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGACCAC302320.17320997641795902Clontech Universal Primer Mix Long (100% over 23bp)
CTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGACTCACTATAGG286080.16390549766356746No Hit
CGTATTAGTCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAG285990.16385393343401725Clontech Universal Primer Mix Long (95% over 24bp)
CGTATTAGATCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTA269220.15424579866116342Clontech Universal Primer Mix Long (100% over 22bp)
CGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAT258740.14824143059798464Clontech Universal Primer Mix Long (95% over 23bp)
TAGTGAGTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCTATAGTGAG254270.1456804071969914No Hit
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGACTAATA249040.1426839525242409Clontech Universal Primer Mix Long (95% over 23bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGTCTAATA233670.13387792798883463Clontech Universal Primer Mix Long (95% over 23bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAA232600.13326488659307112Clontech Universal Primer Mix Long (95% over 23bp)
ATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGAC229380.13142003304694178No Hit
GGTCGCGGCCGAGGATCCTAATACGACTCACTATAGGGCAGCGTGGTCGC227710.13046323012084365Clontech Universal Primer Mix Long (95% over 23bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATTAATACG227160.13014811538470353Clontech Universal Primer Mix Long (95% over 23bp)
GCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGG225870.129409028094484Clontech Universal Primer Mix Long (95% over 23bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATACTAATA220840.12652716059851174Clontech Universal Primer Mix Long (95% over 23bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAACCTAATA218450.125157843836012Clontech Universal Primer Mix Long (95% over 23bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATAGATCGG216200.12386873809725701Clontech Universal Primer Mix Long (95% over 23bp)
CCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCTATA213740.1224593158228849Clontech Universal Primer Mix Short (100% over 21bp)
CGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGC213240.12217284788093935Clontech Universal Primer Mix Long (100% over 23bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAATCTAATA212420.12170304045614863Clontech Universal Primer Mix Long (95% over 23bp)
CCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGA208240.1193081684614838Clontech Universal Primer Mix Long (95% over 23bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAACTAATA202600.11607681007633798Clontech Universal Primer Mix Long (95% over 23bp)
ACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGACTCACT199680.11440383729537594No Hit
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGGCTAAT199610.11436373178350358Clontech Universal Primer Mix Long (95% over 23bp)
CACTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGACTCACTATA198720.11385381884684048No Hit
CCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGAC196720.11270794707905828Clontech Universal Primer Mix Long (95% over 24bp)
CACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGACCACGCTG196210.11241574977827382Clontech Universal Primer Mix Long (100% over 23bp)
CTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGGTCCTCGGCC195420.11196313042999985Clontech Universal Primer Mix Long (100% over 23bp)
GGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAG194990.11171676799992666Clontech Universal Primer Mix Long (95% over 23bp)
GGTCGCGGCCGAGGATCTAATACGACTCACTATAGGGCTAATACGACTCA192170.11010108880735374Clontech Universal Primer Mix Long (95% over 24bp)
ATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGACTCACTATAGGGC192000.11000368970709225Clontech Universal Primer Mix Short (100% over 22bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGAGATCGGAAG189150.10837082243800261Clontech Universal Primer Mix Long (95% over 23bp)
TCGCGGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCGCGGC178220.10210863322707284Clontech Universal Primer Mix Long (95% over 23bp)
CTGCCCTATAGTGAGTCGTATTAGTTCCTCGGCCGCGACCACGCTGCCCT177290.10157580285505409Clontech Universal Primer Mix Long (95% over 24bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGGTCCTCGG176290.101002866971163Clontech Universal Primer Mix Long (100% over 23bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGACCCTAATA174670.1000747108392594Clontech Universal Primer Mix Long (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)