FastQCFastQC Report
Sat 15 Mar 2025
HNVHVAFX7_n02_DAM-GR-bZIP1-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNVHVAFX7_n02_DAM-GR-bZIP1-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13562475
Sequences flagged as poor quality0
Sequence length76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATAC10096737.444607271165476Clontech Universal Primer Mix Long (95% over 23bp)
GGTCGCGGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCGCG4672343.445049668294319Clontech Universal Primer Mix Long (95% over 23bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAACTAATAC1799001.3264540579798303Clontech Universal Primer Mix Long (95% over 23bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGACCTAATAC1491161.0994748377416363Clontech Universal Primer Mix Long (95% over 23bp)
CTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCT1327420.9787446612804815Clontech Universal Primer Mix Long (95% over 24bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGG1190530.8778117563350347Clontech Universal Primer Mix Long (100% over 23bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGCTAATAC1118820.8249379261528593Clontech Universal Primer Mix Long (95% over 23bp)
CTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCC953970.7033893150033457Clontech Universal Primer Mix Long (100% over 23bp)
CGGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGA824270.6077578023185296Clontech Universal Primer Mix Long (95% over 23bp)
GTCGCGGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCGCGG715800.5277797747092621Clontech Universal Primer Mix Long (95% over 23bp)
CAGCGTGGTCGCGGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTG696700.5136967994411049Clontech Universal Primer Mix Long (95% over 23bp)
CCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGACCACGCT579800.4275030921715985Clontech Universal Primer Mix Long (95% over 24bp)
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGC554700.40899614561501496Clontech Universal Primer Mix Long (95% over 24bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATCCTAATA549220.4049555851715856Clontech Universal Primer Mix Long (95% over 23bp)
CGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGACC525510.38747352529682083Clontech Universal Primer Mix Long (100% over 23bp)
CGTGGTCGCGGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTC524230.38652974475528984Clontech Universal Primer Mix Long (95% over 23bp)
CGCGGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCC499920.3686052877516825Clontech Universal Primer Mix Long (95% over 23bp)
CGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGACCAC479560.3535932785129558Clontech Universal Primer Mix Long (100% over 23bp)
AATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGA466380.34387528824937924Clontech Universal Primer Mix Long (95% over 21bp)
GTATTAGATCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAG461050.3399453270881605Clontech Universal Primer Mix Long (100% over 23bp)
CTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGACTCACTA460610.33962090252700927No Hit
ATAGTGAGTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCTATAGTGA419200.30908812735138685No Hit
CGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGACTCA410490.30266599569768793No Hit
ATCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCG407950.30079318118558745Clontech Universal Primer Mix Long (95% over 24bp)
GCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGG382450.2819913032097755Clontech Universal Primer Mix Long (95% over 23bp)
CGTATTAGATCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTA343900.2535672876816363Clontech Universal Primer Mix Long (100% over 22bp)
CGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAT335110.2470861697440917Clontech Universal Primer Mix Long (95% over 23bp)
GTGAGTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTC333190.24567049893179527No Hit
GGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAG323760.2387174907234852Clontech Universal Primer Mix Long (95% over 23bp)
CGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGC321460.23702163506292176Clontech Universal Primer Mix Long (100% over 23bp)
CCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGAC312720.23057738355278073Clontech Universal Primer Mix Long (95% over 24bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATTCTAATA312200.23019397270778377Clontech Universal Primer Mix Long (95% over 23bp)
CCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGA307230.22652944982387066Clontech Universal Primer Mix Long (95% over 23bp)
CACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGACCACGCTG282240.20810360940757494Clontech Universal Primer Mix Long (100% over 23bp)
CTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGACTCACTATAGG279840.2063340208922044No Hit
CCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCTATA269750.19889437584216743Clontech Universal Primer Mix Short (100% over 21bp)
TCGCGGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCGCGGC244290.18012199100827836Clontech Universal Primer Mix Long (95% over 23bp)
GTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTAT242410.17873581333790478No Hit
GGCAGCGTGGTCGCGGCCGAGGATCTAATACGACTCACTATAGGGCAGCG238620.17594133814071547Clontech Universal Primer Mix Long (95% over 23bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGACTAATACG237730.17528511573293223Clontech Universal Primer Mix Long (95% over 23bp)
ACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGACTCACT235670.17376621892390584No Hit
GGTCGCGGCCGAGGATCCTAATACGACTCACTATAGGGCAGCGTGGTCGC233780.17237266796805156Clontech Universal Primer Mix Long (95% over 23bp)
ATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGACTCACTATAGGGC219650.16195421558380754Clontech Universal Primer Mix Short (100% over 22bp)
ATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGAC218790.1613201130324664No Hit
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAATCTAATA214720.1583191858418172Clontech Universal Primer Mix Long (95% over 23bp)
GTGGTCGCGGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCG211970.1562915323346218Clontech Universal Primer Mix Long (95% over 23bp)
CACTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGACTCACTATA206220.15205189318321324No Hit
GGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCG202960.1496482021165016Clontech Universal Primer Mix Long (95% over 24bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGACTCTAATA199440.14705280562729148Clontech Universal Primer Mix Long (95% over 23bp)
TAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACG197550.14565925467143717Clontech Universal Primer Mix Long (95% over 22bp)
GCGGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCG194700.14355786830943468Clontech Universal Primer Mix Long (95% over 23bp)
TAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGACTCACTATAGGGCA185600.13684817852198805Clontech Universal Primer Mix Long (100% over 23bp)
AGCGTGGTCGCGGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGG181880.13410531632316372Clontech Universal Primer Mix Long (95% over 23bp)
CTGCCCTATAGTGAGTCGTATTAGGTCCTCGGCCGCGACCACGCTGCCCT178260.1314361869791465Clontech Universal Primer Mix Long (95% over 24bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGGTCCTCGG173230.12772742438234908Clontech Universal Primer Mix Long (100% over 23bp)
CTGCCCTATAGTGAGTCGTATTAGTTCCTCGGCCGCGACCACGCTGCCCT172850.12744723953408207Clontech Universal Primer Mix Long (95% over 24bp)
ATTAGATCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAT165850.1222859396975847Clontech Universal Primer Mix Long (100% over 23bp)
GCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGA160730.11851081753146088Clontech Universal Primer Mix Long (100% over 23bp)
AGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGACTCACTATAGGGCAG160680.11847395110405734Clontech Universal Primer Mix Long (95% over 23bp)
AGTGAGTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCTATAGTGAGT157020.11577532861811726No Hit
CCTATAGTGAGTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCTATAG150220.1107614944912341No Hit
CGTGGTCGCGGCCGAGGAACTAATACGACTCACTATAGGGCAGCGTGGTC149120.10995043308835593Clontech Universal Primer Mix Long (95% over 23bp)
CGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGACCACGCTGCC148560.10953752910143613Clontech Universal Primer Mix Long (95% over 24bp)
ACTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGACTCACTATAG147390.10867485470019299No Hit
CAGCGTGGTCGCGGCCGAGGAACTAATACGACTCACTATAGGGCAGCGTG146660.10813660486010113Clontech Universal Primer Mix Long (95% over 23bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATAA145940.10760572830548996Clontech Universal Primer Mix Long (95% over 23bp)
AGTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTA145080.10697162575414887No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGTTCCTCGG143560.10585088636108084Clontech Universal Primer Mix Long (100% over 23bp)
CTATAGTGAGTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCTATAGT141570.10438360255041945No Hit
CTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGGTCCTCGGCC138680.1022527230464941Clontech Universal Primer Mix Long (100% over 23bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)