FastQCFastQC Report
Sat 15 Mar 2025
HNVHVAFX7_n02_DAM-GR-bZIP1-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNVHVAFX7_n02_DAM-GR-bZIP1-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13028867
Sequences flagged as poor quality0
Sequence length76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGACTAATACG7842886.019617822486023Clontech Universal Primer Mix Long (95% over 23bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAATACGACT4047663.1066860994129417Clontech Universal Primer Mix Long (95% over 23bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATAC3339952.5634999574406585Clontech Universal Primer Mix Long (95% over 23bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGA2443201.875220615883177Clontech Universal Primer Mix Long (95% over 23bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATAATACGA1548941.1888524151793092Clontech Universal Primer Mix Long (95% over 23bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATTAATACG1235280.9481100697397555Clontech Universal Primer Mix Long (95% over 23bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGA1147940.8810743098382999Clontech Universal Primer Mix Long (100% over 23bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGAGATCGGAAG1110290.8521769390999233Clontech Universal Primer Mix Long (95% over 23bp)
CTAATACGACTCACTATAGGGCCGCGACCACGCTGCCCTATAGAGATCGG1082320.8307092243707761Clontech Universal Primer Mix Short (100% over 22bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATACGACTC1060000.8135780340685035Clontech Universal Primer Mix Long (95% over 23bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAATACGAC959250.7362497445096339Clontech Universal Primer Mix Long (95% over 23bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGCTAATACGA894900.6868594176300978Clontech Universal Primer Mix Long (95% over 23bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAACTAATAC763790.5862290251331908Clontech Universal Primer Mix Long (95% over 23bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGATCGGAA700300.5374987709982764Clontech Universal Primer Mix Long (95% over 23bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGCTAATAC694680.5331852723648188Clontech Universal Primer Mix Long (95% over 23bp)
CTAATACGACTCACTATAGGGCCGCGACCACGCTGCCCTATAGTGAGATC648990.49811698899067736Clontech Universal Primer Mix Short (100% over 22bp)
CTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGAAG627400.4815460929948859Clontech Universal Primer Mix Long (100% over 23bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGACCTAATAC606260.4653205839003499Clontech Universal Primer Mix Long (95% over 23bp)
ATAGTGAGTCGTATTAGTCCTCGGCCGCGACCACGCTGCCCTATAGTGAG506130.3884681607387657No Hit
CTAATACGACTCACTATAGGGCCGCGACCACGCTGCCCTATAGTGAGAGA496900.38138389163079184Clontech Universal Primer Mix Short (100% over 22bp)
AGTGAGTCGTATTAGTCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTC488580.3749980715898013No Hit
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGACTATAGGG487830.3744224267543755Clontech Universal Primer Mix Long (95% over 23bp)
CTGCCCTATAGTGAGTCGTATTAGTCCTCGGCCGCGACCACGCTGCCCTA478810.3674993382003209Clontech Universal Primer Mix Long (95% over 24bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGTCCTCGGC426330.32721955024945765Clontech Universal Primer Mix Long (100% over 23bp)
CTCACTATAGGGCAGCGTGGTCGCGGCCGAGGACTAATACGACTCACTAT420980.3231132837567534No Hit
CTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGTCCTCGGCCG408050.3131891667940121Clontech Universal Primer Mix Long (100% over 23bp)
CTGCCCTATAGTGAGTCGTATTCCTCGGCCGCGACCACGCTGCCCTATAG358890.27545756664796717Clontech Universal Primer Mix Long (95% over 22bp)
CTATAGGGCAGCGTGGTCGCTGCCCTATAGTGAGTCGTATTAGAGATCGG328240.2519328810402317Clontech Universal Primer Mix Long (95% over 24bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGAGATCGGAAGAGCGTCGT314110.24108773234080907Clontech Universal Primer Mix Long (95% over 23bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGAGATCGGAAGAGCGTC311710.23924566886744641Clontech Universal Primer Mix Long (95% over 23bp)
GGTCGCGGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCGCG307800.23624464045875976Clontech Universal Primer Mix Long (95% over 23bp)
CTAATACGACTCACTATAGGGCAGCGTGGAGATCGGAAGAGCGTCGTGTA300200.23041143945977804Clontech Universal Primer Mix Long (95% over 23bp)
CTAATACGACTCACTATAGGGCCGCGACCACGCTGCCCTATAGTAGATCG298500.22910664449947948Clontech Universal Primer Mix Short (100% over 22bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGGCTAATACG293790.2254915949330053Clontech Universal Primer Mix Long (95% over 23bp)
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGAA290060.22262872128482086Clontech Universal Primer Mix Long (95% over 24bp)
CGTATTAGTCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAG278280.21358725973639917Clontech Universal Primer Mix Long (95% over 24bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATAGATCGG265470.20375524594732605Clontech Universal Primer Mix Long (95% over 23bp)
AGTGAGTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCTATAGTGAGT262440.2014296408122057No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTCCTCGGCCGC259680.19931126781783862Clontech Universal Primer Mix Long (95% over 21bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGTACGACT255720.19627186308679026Clontech Universal Primer Mix Long (95% over 23bp)
CTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCT242610.1862095913635468Clontech Universal Primer Mix Long (95% over 24bp)
CTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAATACGACTCACTATAGG238570.18310878451671966No Hit
CTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTCCTCGGCCGCGA232890.17874923429642808Clontech Universal Primer Mix Long (95% over 21bp)
CTAATACGACTCACTATAGGGCAGCGTGGCCGCGACCACGCTGCCCTATA223140.17126585143589232Clontech Universal Primer Mix Long (95% over 23bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATCCTAATA218880.1679961887706736Clontech Universal Primer Mix Long (95% over 23bp)
CACTATAGGGCAGCGTGGTCGCTGCCCTATAGTGAGTCGTATTAGAGATC218620.16779663189439264Clontech Universal Primer Mix Long (95% over 24bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGATCGGAAGAG218500.16770452872072453Clontech Universal Primer Mix Long (95% over 23bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGAGATCGGAAGAGCGT206450.15845583503154956Clontech Universal Primer Mix Long (95% over 23bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCCGGCCGAGAGATCGGAAGA202880.15571576561492262Clontech Universal Primer Mix Long (95% over 23bp)
AATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGACTAATACGAC199760.15332108309955117Clontech Universal Primer Mix Long (95% over 21bp)
CCACGCTGCCCTATAGTGAGTCGTATTAGTCCTCGGCCGCGACCACGCTG197410.15151739594855024Clontech Universal Primer Mix Long (95% over 24bp)
CAGCGTGGTCGCGGCCGAGGACTAATACGACTCACTATAGGGCAGCGTGG196410.1507498695013158Clontech Universal Primer Mix Long (95% over 23bp)
CTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCC194440.14923784240026397Clontech Universal Primer Mix Long (100% over 23bp)
CTATAGTGAGTCGTATTAGTCCTCGGCCGCGACCACGCTGCCCTATAGTG193880.14880802758981268No Hit
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGTCCTCGGCC193620.14860847071353173Clontech Universal Primer Mix Long (95% over 24bp)
ATAGTGAGTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCTATAGTGA190080.14589142709032182No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGG189730.14562279283378976Clontech Universal Primer Mix Long (100% over 23bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGTCTAATACG185370.1422763775238476Clontech Universal Primer Mix Long (95% over 23bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCCTCGGCCGCGACCACGCTG182090.13975889077691867Clontech Universal Primer Mix Long (95% over 23bp)
CTATAGGGCAGCGTGGTCGCGGCCGAGGACTAATACGACTCACTATAGGG172650.13251344111502558Clontech Universal Primer Mix Short (100% over 21bp)
CTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGACTCACTA170220.1306483518482459No Hit
CCCTATAGTGAGTCGTATTAGTCCTCGGCCGCGACCACGCTGCCCTATAG164850.12652673482659696Clontech Universal Primer Mix Short (100% over 21bp)
CTCACTATAGGGCAGCGTGGTCGCTGCCCTATAGTGAGTCGTATTAGAGA162660.12484585190715355Clontech Universal Primer Mix Long (95% over 24bp)
CGTGGTCGCGGCCGAGGACTAATACGACTCACTATAGGGCAGCGTGGTCG157290.12072423488550463Clontech Universal Primer Mix Long (95% over 23bp)
CTAATACGACTCACTATAGGGCAGAGATCGGAAGAGCGTCGTGTAGGGAA156410.12004881161193832Illumina Single End PCR Primer 1 (100% over 26bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGAATACGACTC150380.11542062713511467Clontech Universal Primer Mix Long (95% over 23bp)
CGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGAAGAGCGTCGT146940.1127803361566282Clontech Universal Primer Mix Long (100% over 23bp)
CGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGAAGAGCGTC145040.11132203590688276Clontech Universal Primer Mix Long (100% over 23bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATTATAGGG144850.11117620588190823Clontech Universal Primer Mix Long (95% over 23bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATTCTAATA140040.10748440367071058Clontech Universal Primer Mix Long (95% over 23bp)
GTGAGTCGTATTAGTCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCG138380.10621030976830143No Hit
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGACTACGACT135440.10395378201343217Clontech Universal Primer Mix Long (95% over 23bp)
CGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGACTAATACGACTCAC133920.10278714181363582No Hit
CTAATACGACTCACTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT132640.10180470796117574Illumina Single End PCR Primer 1 (100% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)