FastQCFastQC Report
Sat 15 Mar 2025
HNVHVAFX7_n02_DAM-GR-EV-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNVHVAFX7_n02_DAM-GR-EV-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14536792
Sequences flagged as poor quality0
Sequence length76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATAC14203229.770532590684383Clontech Universal Primer Mix Long (95% over 23bp)
GGTCGCGGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCGCG3023982.080225128075025Clontech Universal Primer Mix Long (95% over 23bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAACTAATAC2806231.9304327942506159Clontech Universal Primer Mix Long (95% over 23bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGACCTAATAC2392731.6459821396632763Clontech Universal Primer Mix Long (95% over 23bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAGCTAATAC1757071.208705469542386Clontech Universal Primer Mix Long (95% over 23bp)
CTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCT1184650.8149322078764007Clontech Universal Primer Mix Long (95% over 24bp)
CTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCC991330.6819455076470792Clontech Universal Primer Mix Long (100% over 23bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATCCTAATA869570.5981856244486404Clontech Universal Primer Mix Long (95% over 23bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGG836780.5756290658901909Clontech Universal Primer Mix Long (100% over 23bp)
ATAGTGAGTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCTATAGTGA817830.5625931773667807No Hit
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGACTCTAATA717720.4937265388402063Clontech Universal Primer Mix Long (95% over 23bp)
CAGCGTGGTCGCGGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTG628410.43228932490744865Clontech Universal Primer Mix Long (95% over 23bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGACTAATACG612950.4216542411833367Clontech Universal Primer Mix Long (95% over 23bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATTCTAATA596890.41060641164845724Clontech Universal Primer Mix Long (95% over 23bp)
CCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGC571230.3929546491412961Clontech Universal Primer Mix Long (95% over 24bp)
AATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGA521730.35890311975296885Clontech Universal Primer Mix Long (95% over 21bp)
CGGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGA491340.3379975444375898Clontech Universal Primer Mix Long (95% over 23bp)
CTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGACTCACTA487410.33529405937706197No Hit
GTCGCGGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCGCGG473100.3254500717902547Clontech Universal Primer Mix Long (95% over 23bp)
CCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGACCACGCT470100.32338634273641664Clontech Universal Primer Mix Long (95% over 24bp)
GTGAGTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTC451390.3105155525373136No Hit
CGTGGTCGCGGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTC430410.29608320735413973Clontech Universal Primer Mix Long (95% over 23bp)
AGTGAGTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCTATAGTGAGT428720.29492063998714435No Hit
CGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGACTCA386090.26559505013210616No Hit
GTATTAGATCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAG354180.24364385209611583Clontech Universal Primer Mix Long (100% over 23bp)
CGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGACC351870.24205478072466058Clontech Universal Primer Mix Long (100% over 23bp)
CGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGACCAC332030.22840665258194517Clontech Universal Primer Mix Long (100% over 23bp)
CGCGGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCC305350.21005322219647912Clontech Universal Primer Mix Long (95% over 23bp)
ATCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCG288350.1983587575580637Clontech Universal Primer Mix Long (95% over 24bp)
CGTATTAGATCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTA279780.19246337156093313Clontech Universal Primer Mix Long (100% over 22bp)
CTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGACTCACTATAGG256100.17617367022930505No Hit
CGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAT248180.1707254255271727Clontech Universal Primer Mix Long (95% over 23bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAATCTAATA240850.16568304753896182Clontech Universal Primer Mix Long (95% over 23bp)
CGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGC236450.16265624492666608Clontech Universal Primer Mix Long (100% over 23bp)
TAGTGAGTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCTATAGTGAG230980.15889337895183478No Hit
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGA220750.15185606287824713Clontech Universal Primer Mix Long (95% over 23bp)
CACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGACCACGCTG219530.15101681306301967Clontech Universal Primer Mix Long (100% over 23bp)
CCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGACCACGCTGCCCTATA212550.14621520346442324Clontech Universal Primer Mix Short (100% over 21bp)
ATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGAC211640.14558920565142572No Hit
ACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGACTCACT209950.1444266382844303No Hit
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATCTCTAAT207880.14300266523728208Clontech Universal Primer Mix Long (95% over 23bp)
GCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGG207690.14287196239720565Clontech Universal Primer Mix Long (95% over 23bp)
CCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGAC206660.1421634154220546Clontech Universal Primer Mix Long (95% over 24bp)
ATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGACTCACTATAGGGC191810.13194795660555644Clontech Universal Primer Mix Short (100% over 22bp)
CCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGA183630.12632085538542479Clontech Universal Primer Mix Long (95% over 23bp)
CACTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACGACTCACTATA181470.12483497046666142No Hit
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGCTAATA178380.12270932954120826Clontech Universal Primer Mix Long (95% over 23bp)
CTGCCCTATAGTGAGTCGTATTAGTTCCTCGGCCGCGACCACGCTGCCCT177650.12220715547144102Clontech Universal Primer Mix Long (95% over 24bp)
GGTCGCGGCCGAGGATCCTAATACGACTCACTATAGGGCAGCGTGGTCGC175870.12098267623283046Clontech Universal Primer Mix Long (95% over 23bp)
GGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAG174040.11972380150998926Clontech Universal Primer Mix Long (95% over 23bp)
CGTATTAGTCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAG174030.11971692241314313Clontech Universal Primer Mix Long (95% over 24bp)
CTGCCCTATAGTGAGTCGTATTAGGTCCTCGGCCGCGACCACGCTGCCCT173600.11942112124875968Clontech Universal Primer Mix Long (95% over 24bp)
GGCAGCGTGGTCGCGGCCGAGGATCTAATACGACTCACTATAGGGCAGCG169330.11648374689546359Clontech Universal Primer Mix Long (95% over 23bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGACCCTAATA167870.1154793987559291Clontech Universal Primer Mix Long (95% over 23bp)
CTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGGTCCTCGGCC162610.11186099381486644Clontech Universal Primer Mix Long (100% over 23bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATAT160720.1105608445109485Clontech Universal Primer Mix Long (95% over 23bp)
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAACCTAATA160690.11054020722041011Clontech Universal Primer Mix Long (95% over 23bp)
TAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGATCTAATACG154940.10658472653388726Clontech Universal Primer Mix Long (95% over 22bp)
TCGCGGCCGAGGATCTAATACGACTCACTATAGGGCAGCGTGGTCGCGGC153920.10588305865558234Clontech Universal Primer Mix Long (95% over 23bp)
CAGCGTGGTCGCGGCCGAGGAACTAATACGACTCACTATAGGGCAGCGTG147150.10122591009075455Clontech Universal Primer Mix Long (95% over 23bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)